Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissueculture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenientsections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular Biology™series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducibleprotocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture. INDICE: 1. An Introduction to Plant Cell Culture Victor M. Loyola-Vargas and Neftalí Ochoa-Alejo 2. History of Plant Tissue Culture Trevor Thorpe 3. Callus, Suspension Culture and Hairy Roots. Induction, Maintenance, and Characterization Rosa M. Galáz-Ávalos, Sagrario Aguilar-Díaz, Pedro A. Xool-González, Silvia M. Huchín-May and Víctor M. Loyola-Vargas 4. Growth Measurements. Estimation of Cell Division and Cell Expansion Gregorio Godoy-Hernández and Felipe A. Vázquez Flota 5. Measurement of Cell Viability Lizbeth A. Castro-Concha, Rosa María Escobedo, and María de Lourdes Miranda-Ham 6. Pathogen and Biological Contamination Management in Plant Tissue Culture: Phyto-Pathogens, Vitro-Pathogens, and Vitro-Pests Alan C. Cassells 7. Cryopreservation of Embryogenic CellSuspensions by Encapsulation-Vitrification and Encapsulation-Dehydration Zhenfang Yin, Long Chen, Bing Zhao, Yongxing Zhu, and Qiaochun Wang 8. The Study of in vitro Development in Plants: General Approaches and Photography Edward C.Yeung 9. Use of Statistics in Plant Biotechnology Michael E. Compton 10. Tissue Culture Methods for the Clonal Propagation and Genetic Improvement of Spanish Red Cedar (Cedrela Odorata) Yuri Peña-Rodríguez, Juan Juárez Gómez, José Antonio González, and Manuel L. Robert 11. Micropropagation of Banana Y?ld?z AkaKaçar and Ben Faber 12. Liquid in vitro Culture for the Propagation of Arundodonax Miguel Ángel Herrera-Alamillo and Manuel L. Robert 13. Production of Haploids and Doubled Haploids in Maize Vanessa Prigge and Albrecht E. Melchinger14. Maize Somatic Embryogenesis: Recent Features to Improve Plant Regeneration Verónica Garrocho-Villegas, Maria Teresa de Jesús-Olivera, and Estela Sánchez Quintanar 15. Improved Shoot Regeneration from Root Explants Using an Abscisic Acid Containing Medium Subramanian Paulraj and Edward C. Yeung 16. Cryopreservation of Shoot Tips and Meristems: An Overview of Contemporary Methodologies Erica E. Benson and Keith Harding 17. Anther Culture of Chili Pepper (Capsicum spp.) Neftalí Ochoa-Alejo 18. Production of Inter-Specific Hybrids in Ornamental Plants Juntaro Kato and Masahiro Mii 19. Plant Tissue Culture of Fast-Growing Trees for Phytoremediation Research José Luis Couselo, Elena Corredoira,Ana M. Vieitez, and Antonio Ballester 20. Removing Heavy Metals by in vitro Cultures María del Socorro Santos-Díaz and María del Carmen Barrón-Cruz 21. Establishment of a Sanguinarine Producing Cell Suspension Culture of Argemone Mexicana L. (Papaveraceae). Induction of Alkaloid Accumulation Felipe A. Vázquez-Flota, Miriam Monforte-González, Cecilia Guízar-González, Jorge Rubio-Piña, and Karen Trujillo-Villanueva 22. Epigenetics, the Role of DNA Methylation on Tree Development Marcos Viejo Somoano, María E. Santamaría, José L. Rodríguez, Luis Valledor, Mónica Meijón, Marta Pérez, Jesús Pascual, Rodrigo Hasbún, MarioFernández Fraga, María Berdasco, Peter E. Toorop, María J. Cañal, and RobertoRodríguez Fernández 23. The Potential Roles of microRNAs in Molecular Breeding Qing Liu and Yue-Qin Chen 24. Determination of Histone Methylation in Mono- and Dicotyledons Plants Geovanny I. Nic-Can and Clelia De la Peña 25. Basic Procedures for Epigenetic Analysis in Plant Cell and Tissue Culture José L. Rodríguez, Jesús Pascual, Marcos Viejo Somoano, Luis Valledor, Mónica Meijón, Rodrigo Hasbún, Norma Yagüe Yrei, María E. Santamaría, Marta Pérez, Mario FernádezFraga, María Berdasco, Roberto Rodríguez Fernández, and María J. Cañal 26. Plant Tissue Culture and Molecular Markers María Tamayo-Ordoñez, Javier Huijara-Vasconselos, Adriana Quiroz-Moreno, Matilde Ortíz-García, and Felipe Sánchez-Teyer 27. Biolistic- and Agrobacterium-Mediated Transformation Protocols for Wheat Cecília Tamáz-Nyitrai, Huw D. Jones, and László Tamás 28. Improved GeneticTransformation of Cork Oak (Quercus suber L.) Rubén Alvarez-Fernández and Ricardo-Javier Ordás 29. Organelle Transformation Anjanabha Bhattacharya, Anish Kumar, Nirali Desai, and Seema Parikh 30. Appendix A - The Components of the Culture Media Víctor M. Loyola-Vargas 31. Appendix B - Plant Biotechnology and Tissue Culture Resources in the Internet Víctor M. Loyola-Vargas
- ISBN: 978-1-61779-817-7
- Editorial: Humana
- Encuadernacion: Cartoné
- Fecha Publicación: 31/05/2012
- Nº Volúmenes: 1
- Idioma: Inglés