B12 Enzyme, Volume 667 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting timely, interesting chapters, including Part I: B12 Biosynthesis and B12 Chaperones, Probing the preference and regioselectivity of lower ligand activation and its role in cobamide diversity, Biosynthesis of corrinoids, The human B12 trafficking chaperones: CblC, CblD and ATR, A method for the efficient adenosylation of corrinoids, Production, purification and liposome reconstitution of cobamide synthase, Extraction and Purification of Cobamides from Bacterial Cultures, Part II: The many faces of B12-dependent enzyme reactions, Methylcobalamin-dependent methionine synthase, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology serials Includes the latest information on B12 Enzymes INDICE: B12 Biosynthesis and B12 Chaperones 1. Biosynthesis of corrinoids 2. Guardian of cobamide diversity: probing the role of CobT in lower ligand activation in the biosynthesis of vitamin B12 and other cobamide cofactors 3. Purification and Detection of Vitamin B12 Analogs 4. A method for the efficient adenosylation of corrinoids 5. A method for the production, purification and liposome reconstitution of cobamide synthase 6. A method for the Isolation of ?-ribazole from Vitamin B12, and its Enzymatic Conversion to ?-ribazole 5'-phosphate 7. The human B12 trafficking chaperones: CblC, CblD and ATR 8. Synthesis and applications of antivitamin B12 derivatives B12-dependent enzyme reactions: 9. Coenzyme B12-dependent eliminases: Diol and glycerol dehydratases and ethanolamine ammonia-lyase 10. Reactivating chaperones for coenzyme B12-dependent eliminases: Diol and glycerol dehydratases and ethanolamine ammonia-lyase 11. Glutamate mutase and 2-methyleneglutarate mutase 12. Human B12 dependent enzymes: Methionine synthase and methylmalonyl-CoA mutase 13. Heterologous production and biophysical characterisation of catabolic Nitratireductor pacificus pht-3B reductive dehalogenase 14. An unusual light-sensing function for coenzyme B12 in bacterial transcription regulator CarH
- ISBN: 978-0-323-91591-5
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/06/2022
- Nº Volúmenes: 1
- Idioma: Inglés