As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity. Angiogenesis Protocols, Second Edition remains true toits original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro. Thisinformation, however, is expanded to include chapters on circulating endothelial progenitor cells, angiogenic signalling pathways, imaging of angiogenesis,and measurement of tissue blood flow. Written in the Methods in Molecular Biology™ series format, the chapters provide step-by-step laboratory protocols, lists of necessary materials and reagents, and a Notes section, which details tips on troubleshooting and avoiding known pitfalls. Key in vitro and in vivo techniques covering angio- and lymphangiogenesis Accessible to all levels of expertise User-friendly format/layout Helpful notes based on real experience Well-illustrated up-to-date methods from key leaders in field INDICE: From the contents Angiogenic Signalling Pathways.- Imaging Angiogenesis.- Assessing Tumor Angiogenesis in Histological Samples.- Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis.- Vascular Endothelial Cells from Human Micro- and Macro-vessels: Isolation, Characterisation and Culture.- Lymphatic Endothelial Cells: Establishment of Primaries and Characterization of Established Lines.- Isolation, Culture and Characterisation of Vascular Smooth Muscle Cells.- Circulating Endothelial Progenitor Cells (CEPC).- In vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation, Motility, Tubular Differentiation and Proteolysis.- Tube Formation: an In Vitro Matrigel Angiogenesis Assay.- Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring Model.- Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature.
- ISBN: 978-1-58829-907-9
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 370
- Fecha Publicación: 01/10/2008
- Nº Volúmenes: 1
- Idioma: Inglés