After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. InNucleic Acid and Peptide Aptamers: Methods and Protocols, expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology™ series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents,step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Provides an easily accessible reference volume for in vitro selection procedures written by leading researchers in the field Covers a wide range of in vitro selection protocols targeting different molecules Comprehensive guide to identifying your specific target binding aptamer, whether of nucleic acid or peptidic nature Detailed presentation of applications of aptamers INDICE: From the contents Nucleic Acid Pool Preparation and Characterization.- In vitro Selection of ssDNA Aptamers Using Biotinylated Target Proteins.-Isolating Aptamers Using Capillary Electrophoresis-SELEX (CE-SELEX).- In vitro Selection of Allosteric Ribozymes.- Cell-Specific Aptamers for Targeted Therapies.- Aptamers Targeting RNA Molecules.- Fluorescence Correlation Spectroscopy (FCS) Based Characterization of Aptamer Ligand Interaction.- Structural Probing Techniques on Natural Aptamers.- Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallography.- Locked Nucleic Acid Aptamers.- Screening of Novel Inhibitors of HIV-1 Reverse Transcriptase with a Reporter Ribozyme Assay.- Aptamers as Artificial Gene Regulation Elements.- Aptamers and Biosensors.- Nanoparticles/Dip Stick.- In vivo Imaging of Oligonucleotidic Aptamers.- Selection of Peptides Interfering with Protein-Protein Interaction.- In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.
- ISBN: 978-1-934115-89-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 360
- Fecha Publicación: 01/04/2009
- Nº Volúmenes: 1
- Idioma: Inglés