Acting principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligand-activated transcription factors to be a crucial part of biological life. In The Nuclear Receptor Superfamily: Methods and Protocols, expert researchers describe a range of molecular, structural and cell biological techniques currently used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new therapeutic strategies for treating nuclear receptor-associated diseases. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as notes from the experts. Provides an easily accessible reference for a range of biochemical and cell-based methods to study nuclear receptor function Clearstep-by-step comprehensive guides written by experienced researchers in the field Covers cutting-edge methodologies, including ChIP-on-chip, siRNA gene silencing and tissues selective gene knock-outs Details analysis from isolated receptor proteins through to cellular and tissue function INDICE: From the contents Nuclear Receptors: One Big Family.- Methods for Measuring Ligand Dissociation and Nuclear Receptor Turnover in Whole Cells.- Flow Cytometry as a Tool for Measurement of Steroid Hormone Receptor Protein Expression in Leukocytes.- X-ray Crystallography of Agonist / Antagonist-Bound Receptors.- FRAP and FRET Methods to Study Nuclear Receptors in Living Cells.- Receptor-DNA Interactions: EMSA and Footprinting.- Chromatin Immunoprecipitation (ChIP) Methodology and Readouts.- Yeast-Based Reporter Assays for the Functional Characterization of Cochaperone Interactions with Steroid Hormone Receptors.- High Throughput Analysis of Nuclear Receptor-Cofactor Interactions.- Binding Affinity and Kinetic Analysis of Nuclear Receptor/Co-Regulator Interactions Using Surface Plasmon Resonance.- Using RNA Interference to Study Protein Function.- Using Intrinsic Fluorescence Emission Spectroscopy to Study Steroid Receptor and Co-Activator Protein Conformation Dynamics.
- ISBN: 978-1-60327-574-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 240
- Fecha Publicación: 01/12/2008
- Nº Volúmenes: 1
- Idioma: Inglés