Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols

Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols is the perfect reference for Bioscience researchers entering the realm of Proteomics. With a strong focus on sample preparation, the book share best practices - collected across a network of key laboratories and core facilities - taking the reader all the way to executing the most usual Mass Spectrometry experiments. In part one, the book provides a guide to extract maximum benefit from the book and an overview of proteomics including a review of exciting research making use of MS proteomics experiments. Part two focuses on the critical sample preparation stage. Chapters cover mammalian cell lines, samples from clinical tissue and biological fluids. A further chapter covers subcellular fractionation. The third part provides methods for protein digestion both for in gel and in solution, including specific methods for Shotgun proteomics. The fourth and final part of the book delves into key MS proteomics analysis protocols including label-free LC-MS, TMT and iTRAQ labelled LC-MS, phosphorylation enrichment, ubiquination enrichment, glycosylation enrichment, LC-MS methods for protein identification. The final chapter covers targeted MS using MRM. All chapters include related analytical software as appropriate. Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols is the perfect lab manual for Proteomic analysis in Biosciences. It can be used both as a reference for research teams or new staff training material. Core facility managers may find it also useful for sharing best practices with their staff and researchers. Explores the most common questions new researchers in this area have Guides readers to properly design the workflow for successful integration of mass spectrometry into protein biochemical analyses Provides examples of sample preparation for a number of different materials, mammalian cells and others INDICE: Section I. Introduction 1. Book introduction 2. MS Proteomic Overview Section II. Sample Preparation 3. Sample preparation from mammalian cell lines 4. Sample preparation for proteomics and MS from clinical tissue 5. Sample preparation for proteomics and MS from biological fluids, e.g. serum/plasma from patient blood 6. Sample preparation for proteomics and MS from bacterial and fungal samples 7. Sample preparation for proteomics and MS from urine samples including exosome isolation 8. Sample preparation for proteomics and MS from animal samples 9. Subcellular fractionation methods for proteomic analysis Section III. Protein Digestion 10. In gel digestion for mass spectrometry 11. In solution digestion for mass spectrometry Section IV. MS Proteomic Analysis Protocols 12. Protocols for Label-free LC-MS/MS proteomic analysis 13. Protocols for TMT labelled LC-MS/MS analysis 14. Phosphorylation enrichment for phosphoproteomic analysis 15. Ubiquitination enrichment for ubiquitinome PTM analysis 16. Glycosylation enrichment and analysis 17. LC-MS for protein identification and PTM analysis (e.g. methylation)

• ISBN: 978-0-323-90395-0
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 350
• Fecha Publicación: 01/08/2023
• Nº Volúmenes: 1
• Idioma: Inglés