Eosinophil Ultrastructure: Atlas of Eosinophil Cell Biology and Pathology

Eosinophil Ultrastructure: Atlas of Eosinophil Cell Biology and Pathology

Melo, Rossana C.N.
Dvorak, Ann M.
Weller, Peter F.

89,39 €(IVA inc.)

Eosinophil Ultrastructure: Atlas of Eosinophil Cell Biology and Pathology is entirely focused on eosinophils and their functional roles in inflammation, host defense and normal homeostatic activities. This book is intended to explore the ultrastructure of human eosinophils, highlighting biological processes observed in normal, experimental and pathological conditions. Created to fill a void in the eosinophil literature, this book has an extensive array of electron microscopic images, which illustrate the diversity of eosinophil morphology. While the atlas is intended primarily as a learning and teaching tool, it is also intended to act as a helpful resource for researchers to identify distinguishing features and structural changes which arise during studies of human eosinophils in culture, after isolation from body fluids or biopsies. Gives guidelines to understand the human eosinophils in studies focused on structural biology, cellular immunology, innate and adaptive immunity, immune responses to pathogens, immunopathology, and inflammatory responsesProvides a core of essential knowledge to identify both immature as mature eosinophilsComprises a representative compilation of the eosinophil ultrastructure during biological processes, such as activation and degranulation, observed mostly in experimental conditionsHighlights eosinophil biological processes found in vivo during human diseases thus providing a link between basic science and clinical aspectsHelps to identify distinguishing features and structural changes which arise during studies of human eosinophils in culture, after isolation from body fluids or biopsies INDICE: Part 1 - The Cell Biology of Human Eosinophils Sections Aspects to be explored 1.1 General Morphology 1.1.1 Immature Eosinophils Early eosinophilic myelocyte Late eosinophilic myelocyte Eosinophilic myelocyte in mitosis 1.1.2 Mature Eosinophils General view Morphology of typical organelles and structures in high magnification: secretory granules, lipid bodies (LBs), Eosinophil Sombrero Vesicles (EoSVs); Views in intact cells and after isolation Cell surface morphology/ CD9 immunolabeling Multivesicular bodies (MVBs) Golgi (normal and Brefeldin A-disrupted) Peripheral Endoplasmic Reticulum (ER) Glycogen granules Organelle interactions: LB-ER; EoSV-granule 1.2 Eosinophil Biological Processes 1.2.1 Activation Increased number of LBs, specific granules changes (number and/or structure), increased number of EoSVs, shape changes 1.2.2 Migration 1.2.3 Secretion/Degranulation Constitutive secretion Classical exocytosis Piecemeal degranulation Cytolytic degranulation Secretion of extracellular vesicles Images from stimulus-induced secretion: A23187, CCL5, CCL11, INF-g, TNF-a, SCF, IgE Immuno-EM for cytokines, tetraspanins and cationic proteins Diaminobenzidina stained eosinophils 1.2.4 Vesicular Trafficking Structural aspects of the vesicular system EoSVs Immuno-EM for 5-LO, CD63, STX17, IL-4, IL-4R, PDI 1.2.5 Cell Death Apoptosis, Necrosis (Cytolysis), EETosis EOSINOPHIL ULTRASTRUCTURE Part 2 - Selected Eosinophil-Associated Diseases Sections Aspects to be explored: biological processes shown above will be highlighted during human diseases. 2.1 Allergies 2.2 Gastrointestinal disorders Inflammatory Bowel Disease Crohn's disease 2.3 Hypereosinophilic syndrome (HES) 2.4 Skin disorders 2.5 Tumors

  • ISBN: 978-0-12-813755-0
  • Editorial: Academic Press
  • Encuadernacion: Rústica
  • Páginas: 300
  • Fecha Publicación: 01/06/2020
  • Nº Volúmenes: 1
  • Idioma: Inglés