Ion Channels, Part C, Volume 653 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Nonsense suppression in ion channels, Engineering Ion Channels Using Protein Trans-splicing, Probing Ion Channel Neighborhoods Using APEX, STX based probes for NaVs, ANAP: a versatile, fluorescent probe of ion channel gating and regulation, High Throughput Screens for Small Molecule Ion Channel Modulators, Using toxins to study ion channels, Re/de-constructing ubiquitin regulation of ion channels, Tethered Peptide Toxins for Ion Channels, Voltage-Sensing Phosphatase Molecular Engineering, and more. Additional chapters cover Engineering excitable cells, Stretch and Poke Stimulation of Mechanically-Activated Ion Channels, Optical Control of STIM Channels, High Throughput Electrophysiological Evaluation of Mutant Ion Channels, Evaluating BEST1 Mutations in RPE Stem Cells, Long Read Transcript Profiling of Ion Channel Splice Variants, Permeation of Connexin Channels, Ratiometric pH indicator for melanosomes and lysosomes, and Ion channels in the epithelial cells of the choroid plexus. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology series INDICE: 1. Nonsense suppression in ion channels Chris Ahern 2. Engineering Ion Channels Using Protein Trans-splicing Stephan Alexander Pless 3. Probing Ion Channel Neighborhoods Using APEX Steven Marx 4. STX based probes for NaVs Justin Du Bois 5. ANAP: a versatile, fluorescent probe of ion channel gating and regulation Michael Puljung 6. High Throughput Screens for Small Molecule Ion Channel Modulators Charles David Weaver 7. Using toxins to study ion channels Eitan Reuveny 8. Re/de-constructing ubiquitin regulation of ion channels Henry M. Colecraft 9. Tethered Peptide Toxins for Ion Channels Steven A. N. Goldstein 10. Voltage-Sensing Phosphatase Molecular Engineering Yasushi Okamura 11. Engineering excitable cells Nenad Bursac 12. Stretch and Poke Stimulation of Mechanically-Activated Ion Channels Jörg Grandl 13. Optical Control of STIM Channels Yubin Zhou 14. High Throughput Electrophysiological Evaluation of Mutant Ion Channels Alfred L. George Jr. 15. Evaluating BEST1 Mutations in RPE Stem Cells Ting Ting Yang 16. Long Read Transcript Profiling of Ion Channel Splice Variants. Elizabeth Tunbridge 17. Permeation of Connexin Channels Jorge E. Contreras 18. Ratiometric pH indicator for melanosomes and lysosomes Elena Oancea 19. Ion channels in the epithelial cells of the choroid plexus Polina V. Lishko
- ISBN: 978-0-12-823924-7
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 18/06/2021
- Nº Volúmenes: 1
- Idioma: Inglés