Essentials in Modern HPLC Separations, Second Edition discusses the role of separation in high performance liquid chromatography (HPLC). This new and updated edition systematically presents basic concepts as well as new developments in HPLC. Starting with a description of basic concepts, it provides important guidance for the practical utilization of various HPLC procedures, such as the selection of the HPLC type, proper choice of the chromatographic column, selection of mobile phase and selection of the method of detection, all of which are in correlation with the physico-chemical characteristics of the compounds separated. Every chapter has been carefully reviewed, with several new sections added to bring the book completely up-to-date. Hence, it is a valuable reference for students and professors in chemistry. Provides a thoroughly updated resource, with an entirely new section on Computer-aided Method Development in HPLC and new subsections on miniaturization and automation in HPLC, chemometric aspects of HPLC, green solvent use in HPLC, and more Includes insights into the chromatographic process to find the optimum solution for analyzing complex samples Presents a basis for understanding the utilization of modern HPLC for applications, particularly for the analysis of pharmaceutical, biological, food, beverage and environmental samples INDICE: Part 1. Basic information about HPLC 1. Introductory information regarding HPLC 2. Overview of HPLC instrumentation and its use 3. Parameters for the characterization of HPLC separation 4. Equilibrium types in HPLC 5. Intermolecular forces 6. Characterization of analytes and matrices 7. Mobile phases and their properties 8. Analytical HPLC columns and their characteristics Part 2. Main types of HPLC separations 9. Reversed-phase HPLC 10. Other HPLC separations performed on hydrophobic stationary phases 11. Hydrophilic interaction chromatography (HILIC) 12. Other HPLC separations performed on polar stationary phases 13. Ion-exchange, ion-moderated, and ligand-exchange liquid chromatography 14. Chiral HPLC Separations 15. Size exclusion HPLC 16. Affinity, immunoaffinity, and aptamer type HPLC 17. Mixed mode HPLC Part 3. Practice of HPLC analysis 18. Utilization of HPLC in chemical analysis
- ISBN: 978-0-323-91177-1
- Editorial: Elsevier
- Encuadernacion: Rústica
- Fecha Publicación: 01/06/2022
- Nº Volúmenes: 1
- Idioma: Inglés