Clostridium difficile , a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible andfrequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In Clostridium difficile: Methods and Protocols ,expert researchers bring together the most recently developed methods for studying the organism, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the use of animal models. Written in thehighly successful Methods in Molecular Biology ™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols serves as an ideal guide for scientists now in a position to gain an in-depth understanding of how this organism is transmitted and how it causes disease. Serves as a comprehensive guide to most of the current techniques for manipulatingand analyzing C. difficile Written by scientists who have pioneered modern C.difficile research Brings together in one place chapters from clinicians, clinical laboratory scientist, and basic scientists to provide a comprehensive understanding of the practical state of the art in C. difficile research INDICE: Clostridium difficile: No Longer an Enigmatic Pathogen?.- Clostridium difficile and the Disease It Causes.- Clostridium difficile Isolation and Culture Techniques.- Molecular Typing Methods for C. difficile: Pulsed-Field Gel Electrophoresis and PCR Ribotyping.- Clostridium difficile Toxinotyping.- Multilocus Sequence Typing for Clostridium difficile.- Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin Genes.- Dissecting the Cell Surface.- Human Intestinal Epithelial Response(s) to Clostridium difficile.- Comparative Genome Analysis of Clostridium difficile Using DNA Microarrays.- ClosTron-Targeted Mutagenesis.- Methods for Gene Cloning and TargetedMutagenesis.- Transposon Mutagenesis in C. difficile.- Refinement of the Hamster Model of Clostridium difficile Disease.- Methods for Working with the Mouse Model.
- ISBN: 978-1-60327-364-0
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 216
- Fecha Publicación: 29/05/2010
- Nº Volúmenes: 1
- Idioma: Inglés