Correlative Light and Electron Microscopy IV

Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more. Contains contributions from experts in the fieldCovers topics using nano-SIMS and EDX for CLEMPresents recent advances and currently applied correlative approachesGives detailed protocols, allowing for the application of workflows in one's own laboratory settingCovers CLEM approaches in the context of specific applicationsAims to stimulate the use of new combinations of imaging modalities INDICE: 1. Workflow (dis)-advantages / spiderweb Ori Avinoam 2. Serial section LM + EM Erin M. Tranfield 3. Platinum clusters as CLEM probes Paul Verkade 4. Correlative Light Electron Microscopy with a transition metal complex as a single probe Paul Verkade 5. Detection EDX + EELS Ben N. G. Giepmans 6. SEM-TEM-SIMS Louise Jensen 7. HPF-CLEM Xavier Heiligenstein 8. A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes Thomas Müller-Reichert 9. Three-dimensional on-section correlative light and electron microscopy of large cellular volumes using STEM tomography Korbinian Buerger 10. Accelerated procedure for approaching and imaging of optically branded ROI in tissue Natalia V. Gounko 11. Volume-CLEM Kedar Narayan 12. Cryo SOFI CLEM Rainer Kaufman 13. Cryo fluo after Cryo lamella Petr Chlanda 14. Super resolution CLEM Sílvia Pujals 15. FIB-SEM + software Allon Weiner 16. Correlia, CLEM software Matthias Schmidt 17. Correlated Multimodality Imaging beyond CLEM: From in-vivo Preclinical Imaging to ex-vivo Microscopy Andreas Walter 18. Community efforts Paul Verkade

• ISBN: 978-0-12-822058-0
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 362
• Fecha Publicación: 01/03/2021
• Nº Volúmenes: 1
• Idioma: Inglés