Major advancements in the field of in situ molecular pathology have occurred since publication of the first edition. In Situ Molecular Pathology and Co-Expression Analyses, Second Edition continues to teach both the molecular basis for the improvements and the actual protocols. This is the unique feature that separates it from the pack of other cook-book type approaches. The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. The automated platforms require that researchers and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols, and regimes for troubleshooting in order to maximize the use of these platforms. This second edition focuses attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. Practicing molecular pathologists, clinical chemists, and toxicologists, as well as clinicians and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived from in situ hybridization and immunohistochemistry. Explains the theory and foundation of immunohistochemistry and in situ hybridization and presents over 200 easy-to-follow experimental protocols with tricks of the tradeIncludes three new chapters: Biochemical basics of improvements for in situ hybridization; Biochemical basics of improvements for immunohistochemistry; Simplified quality control for immunohistochemistry and in situ hybridization: How to know when there is a problemIncludes chapter-ending summaries of Key Points to Remember, bringing beginners up to speed with any seasoned veteran in the fieldThoughtfully tackles the molecular basis if IHC and ISH, along with application of that knowledge to improving the techniques is significant INDICE: 1. Introduction 2. The Basics of Molecular Pathology 3. The Biochemical Basis of In Situ Hybridization and Immunohistochemistry 4. The Basics of In Situ Hybridization 5. The Basics of Immunohistochemistry 6. The Basics of Histologic Interpretations of Tissues 7. The Recommended Protocol for In Situ Hybridization 8. The Recommended Protocol for Immunohistochemistry 9. Co-expression Analyses 10. PCR In Situ Hybridization and RT In Situ PCR
- ISBN: 978-0-12-820653-9
- Editorial: Academic Press
- Encuadernacion: Rústica
- Páginas: 400
- Fecha Publicación: 01/09/2020
- Nº Volúmenes: 1
- Idioma: Inglés