With the great cellular and therapeutic importance of plasma membrane proteins, unbiased technologies such as proteomics become even more vital as they have the power to define patterns of membrane protein expression characteristic of distinct states of cellular development, differentiation or disease, and thereby identify novel markers of, or targets for intervention in, disease. In Membrane Proteomics: Methods and Protocols, leading experts in the field compilea laboratory bench resource which provides a comprehensive toolkit of proven methods. The volume delves into various modifications to standard two-dimensional gel electrophoresis protocols as well as liquid chromatographic methods, protocols for the enrichment of diverse classes of plasma membrane proteins, insilico approaches, and cutting-edge techniques for quantitative membrane protein profiling. Collects together in one volume the diverse practical approaches to analyzing membrane proteins Includes both wet lab and in silico approaches Includes protocols for the isolation of plasma membrane and organellar membrane proteins Covers both prokaryotic and eukaryotic membrane proteins Describes methods useful and accessible to both novice and dedicated proteomic laboratories INDICE: From the contents Section I: In silico Methods for Prediction of Membrane Protein Hydrophobicity and Topology.- Section II: Extraction and Purification of Membrane Proteins. Subsection 1: Plant Membrane Proteins. Subsection 2: Prokaryotic Membrane Proteins. Subsection 3: Mammalian Membrane Proteins.- Section III: Separation of Membrane Proteins.- Section IV: Identification and Quantification of Membrane Proteins.
- ISBN: 978-1-60327-309-1
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 320
- Fecha Publicación: 01/02/2009
- Nº Volúmenes: 1
- Idioma: Inglés