Technical aspects and principles of PCR amplification

Kary Mullis was awarded a Nobel prize for inventing the PCR technique more than a decade ago in 1993. Since its ‘discovery’, multiple adaptations and variations of the standard PCR technique have been described, with many of these adaptations and variations currently being used in clinical diagnostic and research laboratories across the world, from academic laboratories purely interested in basic research to diagnostic laboratories using high throughput routine PCR testing methodologies to detect (for example) minimum residual disease and the presence/absence of particular pathogens. Frequently, PCR technicians limit their understanding of PCR to that particular methodology they are currentlyfamiliar with. However, this approach limits their understanding and appreciation of the range of versatile PCR techniques currently available. Techniques which may be applicable, and indeed more suitable, to their own laboratory situation. Gives a broad overview of available PCR techniques and variants Focussing on the PCR technique, not restricted to a few limited applications associated with a minor discipline Chapters outlining appropriate quality control procedures are included Written by scientists with a broad knowledge of the fieldof PCR techniques and PCR-related clinical research

• ISBN: 978-1-4020-6240-7
• Editorial: Springer
• Encuadernacion: Cartoné
• Páginas: 340
• Fecha Publicación: 01/03/2008
• Nº Volúmenes: 1
• Idioma: Inglés