This manual is a comprehensive compilation of methods that work for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work. Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications.Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America.Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs. INDICE: Foreword by James BatteyForeword by Jeannie FontanaPrefacePart I: Basic methods: Maintenance of stem cell cultures1.Human Embryonic Stem Cell Culture 2.Feeder layers and feeder-free culture3.Mouse Embryonic Fibroblast Feeder Cells 4.Cryopreservation of hESCPart II: Basic methods: Characterization of stem cells5.Classical Cytogenetics: Karyotyping6.Spectral Karyotyping and Fluorescent in situ Hybridization 7.Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns8.FACS/Cell sorting9.Fluorescence Immunocytochemical Analysis of Stem Cells 10.Characterization of Stem Cells Using RT-PCR11.Gene Expression Profiling of Stem Cells by Microarray12.Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice 13.Generation of HESC-Derived Teratomas Part III: Differentiation of stem cells14.Embryoid Body? Formation and Neuroepithelia Differentiation15.Motor Neuron and Dopamine Neuron Differentiation 16.Oligodendrocyte Differentiation from hESC 17.Cardiac 18.Hematopoietic Part IV: Genetic manipulation of stem cells19.Genetic Manipulation of hESC: Lentivirus Vectors 20.Methods developed for mouse ES cellsPart V: Advanced methods21.Derivation of human ES cells22.Neural Stem Cell Culture 23.Transplantation of stem cells24.In vitro fertilization25.Functional characterization-neural cellsPart VI: Practical issues in establishing a human ES cell laboratory26.Setting Up a Facility for hESC Research Part VII: Essays on stem cell biology 27.Intellectual Property: Owning the Stem Cell 28.Ethical Concerns for Stem Cell Research 29.Guidelines for hESC Research Oversight (ESCRO) Committees
- ISBN: 978-0-12-385473-5
- Editorial: Academic Press
- Encuadernacion: Desconocida
- Páginas: 656
- Fecha Publicación: 15/11/2012
- Nº Volúmenes: 1
- Idioma: Inglés