Speed and precision of the diagnostic methods for pathogen detection are often decisive for life and death for patients with rapidly progressing infections such as in sepsis. This outstanding overview sets a new standard for a methodsbook for pathogen detection. The first chapter gives an outline of currently used routine methods including their background, strength and weaknesses. This chapter also serves as a comparison for evaluating the strengths and weaknesses of newer methods. The following chapters cover new methods which are already widely used (PCR and chip–based methods) and which are still more experimental for routine purposes (e.g. spectroscopy). An invaluable resource for all medical laboratories and clinical institutions dealing with infectious diseases. INDICE: 1. Recent identification methods ? an overview (possible authors: Prof. Dr. Michael Bauer, Prof. Dr. Erhard Straube) This chapter provides an overview over the gold standard commonly applied in daily clinical routine, also from a historical view. Here, the chapter will not only focus on bacterial detection but will also include yeasts, molds, parasites and toxins. In addition the chapter will also highlight the limitations of common clinical pathogen detection and defines requirements needed for a more objective, more reliable, more detailed and fast pathogen diagnosis. This chapter will be written by an experienced clinical medical microbiologist. 2. Polymerase chain reaction (PCR) (possible authors: Dr. Marc Lehmann) In order to detect pathogens, polymerase chain reaction (PCR) is one of the most important and applied methods. Within this chapter, the application fields, the challenges of this method (i.e. the design of the primer) and the conceptional realization towards miniaturized and automated set–––ups is introduced. Further, the potential and the trends for future application fields are focused. 3. Chip based methods (possible authors: Dr. Mark Schena) This chapter describes chip based methods applied in bioanalytics. Here, the whole genome analysis via marker analysis up to immunoassays for detection of infection diseases is introduced. Thus, the DNA and RNA detection, as well as the analysis of toxins and other metabolites are focused. Furthermore, preparation and detection methods are critically discussed due to their significance towards a fast and reliable analysis. 4. Trends in on–––site diagnostics (possible authors: PD Dr. Timo Mappes) This chapter deals with new trends in infectognostics concerning the development of robust and miniaturized approaches. These approaches are supposed to pioneer the combination easy and rapid preparation and fast diagnostics and should be path–––breaking for point of care and on–––site diagnostics. 5. MALDI–––ToF and SELDI–––ToF (possible authors: Dr. Peter Lasch) This chapter summarizes the different techniques on mass spectroscopy concerning pathogen as well as toxin detection. Here, the emphasis will be on new technical approaches to minimize sample cultivation and preparation in order to receive fast and reliable results. 6. IR––– and Raman spectroscopy (possible authors: Prof. Dr. Gerwin Pupples) This chapter highlights the recent advances in vibrational spectroscopy which are label free and non–––invasive molecular fingerprint techniques to assess the biochemical composition of pathogens. The potential of these vibrational spectroscopic techniques towards a fast and reliable pathogen diagnosis but also its limitations will be discussed. 7. FACS and FISH (possible authors: Prof. Dr. E. Märtlsbauer) For screening methods both fluorescence activated cell sorting (FACS) and fluorescence in situ hybridization (FISH) were used. Here new methods like PNA FISH will be introduced and discussed regarding a fast clinical diagnosis.
- ISBN: 978-3-527-33516-9
- Editorial: Wiley VCH
- Encuadernacion: Cartoné
- Páginas: 350
- Fecha Publicación: 24/09/2014
- Nº Volúmenes: 1
- Idioma: Inglés