Crop Genome Editing Using CRISPR/Cas9: Theory and Practice

Genome editing is made possible by the use of site-specific nucleases (SSNs) such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPRs). Amongst these SSNs, CRISPR/Cas9 is highly preferred, owing to its simplicity, specificity, versatility and cost effectiveness. By using CRISPR/Cas9, researchers can engineer crops for disease resistance, abiotic stress tolerance and nutritional improvement. Crop Genome Editing Using CRISPR/Cas9: Theory and Practice is highly useful reference for implementing genome engineering technologies, particularly CRISPR related projects in agricultural crops and other plants. This book provides an introduction to CRISPR's basic science and applied aspects along with detailed protocols. It provides detailed a workflow beginning from genome sequence retrieval to mutation analysis in genome edited events using sequencing tools. It helps guide those in the field to plan, design, and conduct experiments methodically. This practical guide aids researchers in accelerating conventional plant breeding programs. Offers a detailed review of literature on genome editing tools with special emphasis on CRISPR/Cas9 and its advancementsContains step-by-step guidelines for single guide RNA design, CRISPR vector construction, protoplast transformation, mutation analysis and Agrobacterium-based regeneration of mutant plantsIncludes detailed troubleshooting tips during various steps INDICE: 1. Introduction to genome editing 2. Crop improvement using CRISPR/Cas9 3. Designing of sgRNA 4. Tools and Resources for the construction of CRISPR vectors 5. Clonin g of CRISPR constructs 6. Protoplast Isolation from plants 7. Delivery of CRISPR constructs into protoplasts and mutation analysis 8. Agrobacterium mediated transformation of CRISPR constructs for the generation of stable mutant plants 9. Recent Advancements in CRISPR/Cas9 genome editing in plants

• ISBN: 978-0-12-816672-7
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 250
• Fecha Publicación: 01/06/2019
• Nº Volúmenes: 1
• Idioma: Inglés