In this fully revised and updated second edition of Bioluminescence: Methods and Protocols, expert researchers contribute a readable and utilitarian compilation of the newest and most innovative techniques that have emerged in this rapidly expanding and progressively diverse field including methods to assess cell trafficking, protein-protein interactions, intracellular signaling, and apoptosis. Also opening up the possibility to visualize and quantify biological mechanisms in real-time and in in vivo settings, the volume also describes thein vivo study of bacterial or viral infections, transplanted cells, stem cells proliferation, vascular flow, and tumors. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials, equipment, and reagents, step-by-step, readily reproducible laboratory protocols, and noteson troubleshooting and avoiding known pitfalls. Provides an easily accessiblereference volume for a wide range of bioluminescence assays and applications written by leading researchers in the field Comprehensive guide to developing,validating, running, and analyzing bioluminescence assays Develops detection and imaging methods Describes the use of a wide variety of bioluminescence tools Presents protocols with enough details to be followed and adapted INDICE: From the contents Luminescent Probes and Visualization of Bioluminescence.- Validation of Bioluminescent Imaging Techniques.- Assessment of Extracellular ATP Concentrations.- High Throughput Quantitative Bioluminescence Imaging for Assessing Tumor Burden.- Fluorescence Imaging of Tumors with "Smart"pH-Activatable Targeted Probes.- Imaging Vasculature and Lymphatic Flow in Mice Using Quantum Dots.- Bioluminescent Imaging of Transplanted Islets.- Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cell Survival, Proliferation, and Fate.- Detection of Apoptosis Using Cyclic Luciferase in Living Mammals.- Non-Invasive Bioluminescent Imaging of Infections.- Real-Time Bioluminescence Imaging of Viral Pathogenesis.- Bioluminescent Monitoring of in vivo Colonization and Clearance Dynamics by Light-Emitting Bacteria.- Quantitative In Vivo Imaging of Non-Viral Mediated Gene Expression and RNAi Mediated Knockdown.- Analysis of Protein-Protein Interactions Using Bioluminescence Resonance Energy Transfer.
- ISBN: 978-1-60327-320-6
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 275
- Fecha Publicación: 01/09/2009
- Nº Volúmenes: 1
- Idioma: Inglés