The aim of Circadian Rhythms is to provide a resource that can be adopted by several types of users: those who are new to circadian biology, those who are already active in the field but are interested in learning new techniques and researchers who are considering moving to a new a model system or undertaking comparative studies and would like to consult protocols applied to different organisms before starting the study of new species. This book features a full range of methods that illustrate procedures that have been recently been introduced in circadian studies and by presenting variations to take into account the peculiarities of different model systems. Methods for analysis of rhythmic readouts in select model organisms. Novel techniques for identifying clock genes by mutagenesis and mircroarrays. Procedures for isolation, extraction, and analysis of RNA. Procedures for extraction, fractionation, and purification of proteins. Detailed in vitro protocols for circadian research. Methods for microscopy analysis in circadian biology. INDICE: From the contents Light, photoreceptors and circadian clocks. Statistical analysis of biological rhythm data. Rhythmic conidiation in Neurosporacrassa. Monitoring and analyzing Drosophila circadian locomotor activity. Automated video image analysis of larval zebrafish locomotor rhythms. Locomotor activity in rodents. Analysis of circadian leaf movement rhythms in Arabidopsisthaliana. Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. Analysis of rhythmic gene expression in adult Drosophila using the firefly-luciferase reporter gene. Monitoring circadian rhythms in Arabidopsis thaliana using luciferase reporter genes. Specialized techniques for site directed mutagenesis in cyanobacteria. Novel strategies for the identificationof clock genes in Neurospora with insertional mutagenesis. Mutagenesis with Drosophila. Mutagenesis in Arabidopsis.-
- ISBN: 978-1-58829-417-3
- Editorial: Humana Press
- Encuadernacion: Cartoné
- Páginas: 578
- Fecha Publicación: 01/01/2008
- Nº Volúmenes: 1
- Idioma: Inglés