Androgens play a critical role in the development and maintenance of the malereproductive system and affect important physiological processes and pathological conditions, including the homeostasis of the normal prostate and prostatecancer. Androgen Action: Methods and Protocols is designed to provide a tool box to study various phases of androgen action, from its entry to the cell to the phenotypic response that the cell mounts, with up-to-date techniques for biochemists, molecular biologists, cell biologists, geneticists, and pathologists. The volume opens with a brief review of the research history on androgen action and prostate carcinogenesis, followed by chapters that cover state-of-the-art methods to determine androgen levels in biological tissues and fluids, experimental procedures to study the various aspects of androgen receptor activity, and methodology to study salient examples of interactions between androgen signalling and other major signalling pathways in cells. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Androgen Action: Methods and Protocols provides a comprehensive overview of, and practicalguidance on, the diverse methodologies that are propelling androgen action research forward, both for normal physiology as well as in disease states. Includes cutting-edge methods and protocols. Provides step-by-step detail essential for reproducible results. Contains key notes and implementation advice from the experts. INDICE: Molecular Mechanisms of Androgen Action – A Historical Perspective. Androgen Action during Prostate Carcinogenesis. Androgen Quantitation in Prostate Cancer Tissue using Liquid Chromatography Tandem Mass Spectrometry. Ligand Competition Binding Assay for the Androgen Receptor. Analysis of Androgen Receptor Activity by Reporter Gene Assays. Identification and Characterization of Androgen Response Elements. In vitro and In vivo Silencing of the Androgen Receptor. Analysis of Interdomain Interactions of the Androgen Receptor. Methods to Study Dynamic Interaction of Androgen Receptor with Chromatin in Living Cells. FRET Analysis of Androgen Receptor Structure and Biochemistry in LivingCells. Analysis of Nuclear Receptor Acetylation. Analysis of Androgen Receptor SUMOylation. Analysis of Ligand-specific Co-repressor Binding to the Androgen Receptor. Detection of Ligand-selective Interactions of the Human Androgen Receptor by SELDI-MS-TOF. Global Identification of Androgen Response Elements. Tissue Specific Knockout of Androgen Receptor in Mice. Methodology to Investigate Androgen-sensitive and Castration-resistant Human Prostate Cancer Xenografts in Preclinical Setting. Automated Microscopy and Image Analysis for Androgen Receptor Function. Androgen Regulation of ETS-gene Fusion Transcripts in Prostate Cancer. Regulation of Apoptosis by Androgens in Prostate Cancer Cells. Analysis of Androgen Receptor Rapid Actions in Cellular Signaling Pathways: Receptor /Src Association. Analysis of Androgen-induced Increase in Lipid Accumulation in Prostate Cancer Cells.
- ISBN: 978-1-61779-242-7
- Editorial: Humana Press
- Encuadernacion: Cartoné
- Páginas: 350
- Fecha Publicación: 29/07/2011
- Nº Volúmenes: 1
- Idioma: Inglés