Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions. On the other hand, phospholipases are emerging as useful enzymes in food and pharmaceutical industries. In Lipases and Phospholipases : Methods and Protocols , expert researchers in the field provide key techniques to investigate these essential enzymes. Focusing on fundamental issues, current and new applications as well as practical step-by-step protocols, and the extensive applications of lipases and the potential application of phospholipases and its inhibitors. Written in the highly successful Methods in Molecular Biology ™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Lipases and Phospholipases : Methods and Protocols aids scientists in continuing to study lipases, phopholipases and related enzymes. Details fundamental issues and new applications of lipases and phospholipases. Provides step-by-step detail essential for reproducible results. Containskey notes and implementation advice from the experts. INDICE: Lipases: an Overview.-Lipases or Esterases: Does it Really Matter? Toward a New Bio-Physico-Chemical Classification.-Study of New Feruloyl Esterases to Understand Lipase Evolution: The Case of Bacillus Flexus.-Phospholipases: an Overview.-High-Throughput Screening Method for Lipases/Esterases.-Functional-Based Screening Methods for Lipases, Esterases and Phospholipases in Metagenomic Libraries.-Plant Lipases:Partial Purification of C. papaya Lipase.-Plant Phospholipases: an Overview.-Lipase and Phospholipase Inhibitors: Design and Applications.-High-Throughput Assay of Secreted Phospholipases A2 Inhibitors.-Heterologous Expression Systems for Lipases: A Review. Streptomyces phospholipase D Cloning and Production.-Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses.-Lipases Production by Solid-State Fermentation: The Case of Rhizopus Homothallicus in Perlite.-Lipases and Esterases from Extremophiles: Overview and Case Example of the Production and Purification of an Esterase from Thermus thermophilus HB27.-Purification, Biochemical and Kinetic Properties of Recombinant. Staphylococcus aureus Lipase.-Phospholipase A2 Purification and Characterization; A Case Study.-The Yeast Yarrowia lipolytica as a Generic Tool for Molecular Evolution of Lipases.-Molecular Modeling of Lipase Binding to a Substrate-Water Interface.-Different Strategies for Hyperactivation of Lipase Biocatalysts.-Rational Design of Immobilized Lipases and Phospholipases.-Thermodynamical Methods for the Optimization of Lipase Catalyzed Reactions.-Lipases as Biocatalysts for the Synthesis of Structured Lipids.-Lipase-Catalyzed Modification of Phenolic Antioxidants.-Lipases as Catalysts in Synthesis of Fine Chemicals.-Lipases in Green Chemistry: Acylation and Alcoholysis on Steroids and Nucleosides.-Lipases as Biocatalyst for Biodiesel Production.-Biopolymer Synthesis Catalyzed by Lipases.-Phospholipases in Food Industry -A Review.-Lipase and Phospholipase Biosensors: a Review.
- ISBN: 978-1-61779-599-2
- Editorial: Springer New York
- Encuadernacion: Cartoné
- Páginas: 643
- Fecha Publicación: 31/03/2012
- Nº Volúmenes: 1
- Idioma: Inglés