As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant growssteadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Contains a comprehensive set of methods for developing transgenic maize varieties, including construct design, transformation, analysis, and breeding Includes methods in tissue culture, molecular biology, biochemistry, genetics, and agronomy Presents data from case studies of research with transgenic maize Displays full-color plates to illustrate the utility of fluorescent marker proteins INDICE: Design of Gene Constructs for Transgenic Maize.- Transient Expression of GFP in Immature Seed Tissues.- Biolistic Gun Mediated Maize Genetic Transformation.- Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus.- Whiskers-Mediated Maize Transformation.- Methods for Generation and Analysis of Fluorescent Protein Tagged Maize Lines.- Use of Transgene-Induced RNAi to Regulate Endogenous Gene Expression.- Plasmid Rescue: Recovery of Flanking Genomic Sequences from Transgenic Transposon Insertion Sites.- Non-Radioactive Genomic DNA Blots for Detection of Low Abundant Sequences in Transgenic Maize.- Tissue-Print Immuno-Detection of Transgene Products in Endosperm for High-Throughput Screening of Seeds.- Determination of Transgene Copy Numberby Real-Time Quantitative-PCR.- Herbicide Resistance Screening Assay.- Characterizing Transgene Inheritance.- Backcross Breeding.- Appendix A: Sample APHISNotification of Release of Regulated Material.- Appendix B: Sample SOP for Field Release.
- ISBN: 978-1-934115-49-7
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 210
- Fecha Publicación: 01/02/2009
- Nº Volúmenes: 1
- Idioma: Inglés