Two-dimensional electrophoresis (2DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers examine these cutting edge proteomics methodologies, explore their application to a wide range of biological materials, and look at how these developments have helped standardize the design, execution, and analysis of proteomics experiments. Chapters reflect key steps in 2DE experiments, including sample preparation, staining, post-translational modification, spot identification, and bioinformatics. Covers a wide biological range of proteome sources including microbes, plants and animals Addresses key aspects of experimental design Includes some of the latest approaches such as DIGE and detection of post-translational modification INDICE: From the contents Two Dimensional Electrophoresis (2DE): An Overview.- Solubilisation of Proteins in 2DE: An Outline.- Selection of pH Ranges in2DE.- Difficult Proteins.- Organelle Proteomics.- Applications of Chemical Tagging Approaches in Combination With 2DE and Mass Spectrometry.- Immunoblotting 2DE Membranes.- Troubleshooting Image Analysis in 2DE.- Analysis of Bacterial Proteins by 2DE.- Proteomic Analysis of Caenorhabditis elegans.- Protein Extraction for 2DE.- Analysis of Proteins from Marine Molluscs.- Preparation and Analysis of Plant and Plastid Proteomes by 2DE.- High-Resolution 2DE.- Blue Native Gel Electrophoresis (BN-PAGE) Proteomics .- 2DE for Proteome Analysis of Human Metaphase Chromosomes.- Microsomal Proteomics.- Pre-Fractionation Using Microscale Solution IEF.- Diagonal Electrophoresis for Detection of Protein Disulphide Bridges.- High-Resolution Large Gel 2DE.- Silver-Staining of Proteinsin 2DE Gels.
- ISBN: 978-1-58829-937-6
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 515
- Fecha Publicación: 01/02/2009
- Nº Volúmenes: 1
- Idioma: Inglés