The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossibleto study as recently as ten years ago. This third edition of Protein NMR Techniques, expands upon the previous editions with current, detailed authoritative but down-to-earth descriptions of new methodologies. These include techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing. Written in the highly successful Methods in Molecular Biology™series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproduciblelaboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein NMR Techniques,Third Edition, seeksto aid scientists in understanding the latest innovations in the field of protein NMR. Comprehensive description of the latest innovations in the field of protein NMR. Provides step-by-step detail essential for reproducible results. Contains key notes and implementation advice from the experts. INDICE: A Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-labeled Proteins. Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis. Isotope Labeling in Insect Cells. Isotope Labeling in Mammalian Cells. Cell-free Protein Production for NMR Studies. Cell-free Membrane Protein Expression for Solid-state NMR. Expression and Purification of Src-family Kinases for Solution NMR Studies. NMR Studies of Large Protein Systems. Protein Dynamics by 15N Nuclear Magnetic Relaxation. Bacterial Production and Solution NMR Studies of aViral Membrane Ion Channel. Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy. NMR Studies of Protein-RNA Interactions. Preparation and Optimization of Protein-DNA Complexes Suitable for Detailed NMR Studies. NMR Studies of Protein-ligand Interactions. In-cell NMR Spectroscopy in Escherichia coli. Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS solid-state NMR. Solid-State NMR Spectroscopy of Protein Complexes. Synthesis, Purification and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy. Assignment of Backbone Resonances in aEukaryotic Protein Kinase - ERK2. Electrostatics of Hydrogen Exchange for Analyzing Protein Flexibility. Fast Protein Backbone NMR Resonance Assignment using the BATCH Strategy. Comprehensive Automation for NMR Structure Determination of Proteins. ARIA for Solution and Solid-state NMR Protein NMR Techniques. Determining Protein Dynamics from 15N Relaxation Data by using DYNAMICS.
- ISBN: 978-1-61779-479-7
- Editorial: Humana Press
- Encuadernacion: Cartoné
- Páginas: 639
- Fecha Publicación: 28/01/2012
- Nº Volúmenes: 1
- Idioma: Inglés