Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner to aid in comprehension of this complex subject. Each technique is explained within its conceptual framework to enhance understanding, and current applications of clinical laboratory techniques are covered in detail. The targeted approach covers the principles of molecular biology, including the basic knowledge of nucleic acids, proteins, and chromosomes; the basic techniques and instrumentations that are commonly used in the field of molecular biology with detailed procedures and explanations for each technique; and the applications of the principles and techniques currently employed in the clinical laboratory Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. Diagnostic Molecular Biology describes their genome structure, life cycle, and evolution, as well as the techniques to collect, isolate, and purify viral DNA/RNA. Diagnostic Molecular Biology, Second Edition expands the discussion on NGS application and its role if future precision medicine. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq are added. Provides understanding of how techniques are used to diagnosis at the molecular level Explains how to use information technology to communicate and assess results in the lab Enhances understanding of fundamental molecular biology and places techniques in context Places protocols into context with practical applications Now with extra chapters on respiratory viruses (Corona) INDICE: Part I 1. Nucleic Acids: DNA and RNA 2. Nucleic Acid-Based Cellular Activities 3. Gene Expression: Transcription of the Genetic Code 4. Gene Expression: Translation of the Genetic Code 5. The Genome Part II 6. Extraction and Purification of Nucleic Acids and Proteins 7. Detection and Analysis of Nucleic Acids 8. Quantification and Analysis of Proteins 9. Amplification of Nucleic Acids 10. Characterization of Nucleic Acids and Proteins 11. Techniques in Sequencing 12. Genome and Transcriptome Analysis Part III 13. Molecular Diagnosis of Chromosomal Disorders 14. Molecular Diagnosis of Mutation and Inherited Diseases 15. Molecular Diagnosis of Infectious Diseases 16. Guidance for Molecular Clinical Laboratory
- ISBN: 978-0-323-91788-9
- Editorial: Academic Press
- Encuadernacion: Rústica
- Páginas: 526
- Fecha Publicación: 01/06/2023
- Nº Volúmenes: 1
- Idioma: Inglés