Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Labelling

Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in this series on enzymology Updated release includes the latest information on methods to measure ubiquitin chain length and linkage, genetic approaches to study the yeast ubiquitin system, amongst many other timely topics INDICE: Design of optogenetic proteins David S. Lawrence Application of optogenetic proteins David S. Lawrence Antibody aggregation mechanism probed by a fluorescently-labeled antibody with fluorescence correlation spectroscopy Shinya Honda Bimane labeling of ß-arrestins to measure their interaction with GPCRs Arun K. Shukla Reversible biotinylation of proteins for investigating their interaction with partners Arun K. Shukla Chemical biology approaches to study RNA cytidine acetylation Jordan Meier Salt sensitive intein in robotic production of peptides Shih-Che Sue Isotope-coded Mass Spec Tags to study interfacial protein-membrane interactions Nicole S. Sampson Direct Cloning by Transformation-Associated Recombination and Heterologous Expression of Biosynthetic Gene Cluster Bradley Moore Allosteric protein switches: from rational design to real applications John Karanicolas Recombinant expression of protein kinases and their use for synthetic biology Stefan T. Arold Small molecule target identification using photo-affinity chromatography Timothy W. Corson Studying protein interactions by in-cell NMR Alexander Shekhtman Investigating the role of glycosylation in protein structure, function, and stability Zhongping Tan Drug inducible genome editing Yu Wang Drug inducible genome activation Yu Wang Computation biology towards directed biosynthesis of natural peptide products Shiou-Chuan (Sheryl) Tsai Emerging protein engineering approaches for imaging applications Itaru Hamachi Engineering structure and function of glycosyltransferases Marcelo E. Guerin Using Cas-Targeted m6A reader proteins to study the effects of reader proteins on single RNAs Bryan C. Dickinson Synthetic genetic circuits for regulation of sexual reproduction Shinya Honda

• ISBN: 978-0-12-818119-5
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 01/06/2019
• Nº Volúmenes: 1
• Idioma: Inglés