Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Sensors

Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in this series on enzymologyUpdated release includes the latest information on methods to measure ubiquitin chain length and linkage, genetic approaches to study the yeast ubiquitin system, amongst many other timely topics INDICE: Site-directed ethylation of membrane proteins for measuring conformational transitions in lipid bilayers Gianluigi Veglia Design and Synthesis of Fluorescent Activity Probes for Protein Phosphatases Cliff. I. Stains Utilizing Split-NanoLuc Fragments as Luminescent Probes for Protein Solubility in Living Cells Cliff. I. Stains SH2-domain based shttps://editorial.elsevier.com/app/book?execution=e16s5#ensor for intracellular recognition of sulfo-tyrosine Jiantao Guo DNA-encoded immunoglobulins for detection of parasites Thomas Kodadek An engineered TEV protease - calmodulin fusion based sensor for neuronal calcium recording Scott T. Laughlin Split-enzyme-based novel strategies for calcium recording Scott T. Laughlin Peptide engineering platform technology Weijun Shen High-throughput methods for aptamer discovery and analysis Andrej Luptak PEGylation of proteins and peptides Joshua L. Price Quantifying Drug-Target Engagement in Live Cells Using Sulfonyl Fluoride Chemical Probes Lyn Jones In bulla channel synthesis and functional expression system under applied membrane potentials Shigetoshi Oiki Drop-in-Well Chip for the Droplet Interface Bilayer with built-in electrodes and low background noise Shigetoshi Oiki Engineering protein degradation in mammalian cells Laura Segatori Engineering split proteins for the design of AND gates Laura Segatori Novel sensors for chemical cytometry analysis Nancy Allbritton Synthetic cells to study protein: membrane lipid dynamic interactions Brian Y. Chow Inter-subunit cyclization as an approach to stabilize oligomeric proteins and complexes Xudong Qu Metabolic chemical reporters of post-translational modifications Matthew Pratt Methods for Design, Characterisation and Application of Inducible Gene Expression Systems Naglis Malys

• ISBN: 978-0-12-818117-1
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 01/06/2019
• Nº Volúmenes: 1
• Idioma: Inglés