Integrated Methods in Protein Biochemistry: Part B, Volume 678 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Precise modification of native proteins, purification, and analysis of bioconjugates, NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein ß? subunit and PLC-ß enzyme in PIP2 hydrolysis and downstream signaling, Structure and function of bacterial secretion system, Tools and protocols for probing protein sumoylation, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more. Additional sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Structural and Functional characterization of lytic polysaccharide monooxygenases, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology series Includes the latest information on Integrated Methods in Protein Biochemistry INDICE: 1. Precise modification of native proteins, purification, and analysis of bioconjugates 2. NanoBiT-based methods to monitor the activation and modulation of RTKs 3. The interplay of G-protein ß? subunit and PLC-ß enzyme in PIP2 hydrolysis and downstream signaling 4. Structure and function of bacterial secretion system 5. Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase 6. Tools and protocols for probing protein sumoylation 7. Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase 8. DeGlyPHER: MS-based analysis of viral spike N-glycoforms 9. Covalent protein painting: MS-based protein footprinting 10. Characterization of GPCR signaling complexes using negative-staining electron microscopy 11. Probing protein misfolding and dissociation with free electron laser 12. Optimized protocol for the characterization of Cas12a activities 13. Proximity proteomics for the identification and characterization of extracellular vesicles 14. Structural and Functional characterization of lytic polysaccharide monooxygenases 15. Characterization of RRE domain in RiPP biosynthesis 16. Structural and mechanistic insights into ferrous iron import and export systems 17. Testing anti-cancer drugs with holographic Incoherent light source quantitative phase imaging 18. Gel-based sample preparation methods for top-down native/cross-linking mass spectrometry 19. Identification and characterization of natural products and their interacts with anti-mycobacterial drug target proteins 20. Integrated studies on ageing-related proteins extending life span in yeast 21. An optimized pipeline for global selenoproteome analysis 22. High-throughput screening and evolution of carbohydrate-active enzymes 23. Regulation of enzymes with light using unnatural amino acids 24. An optimized protocol for modular cloning to rapidly assemble expression vectors for MS-based orthogonal interactomics studies 25. Nucleotide-linked resins to identify new nucleotide-binding proteins using proteomics 26. Phospho-proteomic analysis of immune cells expressing chimeric antigen receptors 27. Novel strategies for targeting ubiquitin conjugation machinery using synthetic molecules 28. Reconstitution of the MLL1 complex and probing methyltransferase activity 29. Probing cellular aspects of endocytosis and phagocytosis with super-resolution microscopy 30. Structural and functional analysis of mono-copper enzymes from fungal species
- ISBN: 978-0-323-99264-0
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 10/01/2023
- Nº Volúmenes: 1
- Idioma: Inglés