Integrated Methods in Protein Biochemistry: Part C, Volume 679 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein ß? subunit and PLC-ß enzyme in PIP2 hydrolysis and downstream signaling, Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems, Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more. Other sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Functional characterization of lytic polysaccharide monooxygenases (LPMOs), Characterization of RRE domain in RiPP biosynthesis, The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations, Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging, and more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology serials Updated release includes the latest information on Integrated Methods in Protein Biochemistry INDICE: 1. NanoBiT-based methods to monitor the activation and modulation of RTKs 2. The interplay of G-protein ß? subunit and PLC-ß enzyme in PIP2 hydrolysis and downstream signaling 3. Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems 4. Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase 5. Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase 6. DeGlyPHER: MS-based analysis of viral spike N-glycoforms 7. Covalent protein painting: MS-based protein footprinting 8. Characterization of GPCR signaling complexes using negative-staining electron microscopy 9. Probing protein misfolding and dissociation with free electron laser 10. Optimized protocol for the characterization of Cas12a activities 11. Proximity proteomics for the identification and characterization of extracellular vesicles 12. Functional characterization of lytic polysaccharide monooxygenases (LPMOs) 13. Characterization of RRE domain in RiPP biosynthesis 14. The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations 15. Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging 16. Gel-based sample preparation methods for top-down native/cross-linking mass spectrometry 17. Identification and characterization of natural products and their interacts with anti-mycobacterial drug target proteins 18. Quantitative cysteinome profiling with DIA-ABPP 19. High-throughput screening and evolution of carbohydrate-active enzymes 20. Regulation of enzymes with light using unnatural amino acids 21. An optimized protocol for modular cloning to rapidly assemble expression vectors for MS-based orthogonal interactomics studies 22. Nucleotide-linked resins to identify new nucleotide-binding proteins using proteomics 23. Phospho-proteomic analysis of immune cells expressing chimeric antigen receptors 24. Novel strategies for targeting ubiquitin conjugation machinery using synthetic molecules 25. Reconstitution of the MLL1 complex and probing methyltransferase activity 26. Probing cellular aspects of endocytosis and phagocytosis with super-resolution microscopy 27. Structural and functional analysis of mono-copper enzymes from fungal species 28. Structure, function and regulation of deubiquitinases and RNA-modifying proteins. 29. Electrophysiology of ion-transporting rhodopsins 30. Measuring protein post-translational modifications using the protein semi-synthesis 31. Assay design and analysis of human Uracil DNA Glycosylase (UNG2) 32. Structure and function of T-Cell Protein Tyrosine Phosphatase (TCPTP) 33. Gel-based sample preparation methods for top-down/native/cross-linking mass spectrometry 34. Global substrate profiling of post-translational modifying enzymes 35. Methods for measuring kinetic isotope effects on the hydroxylation of a peptide
- ISBN: 978-0-443-18592-2
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 10/01/2023
- Nº Volúmenes: 1
- Idioma: Inglés