Today’s tissue microarray (TMA) method presents as a modern high-tech technology, one which allows for the linking of clinical data to the tissues that arecombined on one slide. In Tissue Microarrays: Methods and Protocols, expert researchers explore the current world of TMA making and TMA applications, providing insight into the inherent and complex aspects of the most popular assays used for in-situ tissue analysis. Chapters examine the range of TMA techniquesthat allow for a large number of tissues to be included in one TMA, preserve the integrity of donor tissue blocks, and present a highly organized array pattern that allows for the reliable allocation of clinical data to individual tissue spots. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods,a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Contemporary and ground-breaking, Tissue Microarrays: Methods and Protocols serves as an essential handbook for pathologists, molecular biologists, researchers in the life sciences, as well as physicians, a reflection of the various applications of current TMA technology.Expert TMA protocols for all types of tissues including formalin fixed tissues, frozen tissues, and cell suspensions Comprehensive description of in-situ techniques at the DNA, RNA, and protein level Strategies for manual and automated TMA analysis INDICE: Applications of tissue microarray technology.- Quality aspects of TMA analysis.- Representativity of TMA studies.- Recipient block TMA technique.- Protocol for constructing tissue arrays by cutting edge matrix assembly.- Hypodermic needle without recipient paraffin block technique.- Resin Technologies: Constructing and staining of resin TMAs.- Tissue microarrays from frozen tissues - OCT technique.- An Alternative Technology to Prepare Tissue Microarray Using Frozen Tissue Samples.- Building “Tissue” Microarrays from Suspension Cells.- TMAs from Biopsy specimens.- Immunohistochemical analysis of tissue microarrays.- DNA copy number analysis on Tissue Microarrays.- RNA expression analysis on formalin-fixed paraffin-embedded (FFPE) tissue in TMA format by RNA in situ hybridization.- Automated analysis of Tissue Microarrays.- Digital microscopy for boosting database integration and analysis in TMA studies.- From Gene to Clinic: TMA based clinical validation of molecular markers in prostate cancer.
- ISBN: 978-1-60761-805-8
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 193
- Fecha Publicación: 20/08/2010
- Nº Volúmenes: 1
- Idioma: Inglés