Stem cells are found in almost all organisms from the early stages of development to the end of life. There are several types of stem cells and all of them may prove useful for medical research; however, each of the different types has both promise and limitations. Somatic Stem Cells: Methods and Protocols presents selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Chapters focus on the isolation, characterization, purity, plasticity, and clinical uses of somatic stem cells from a variety of human and animal tissues. Written in the highly successfulMethods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Through and intuitive, Somatic Stem Cells: Methods and Protocols seeks to provides scientists with the fundamental techniques of stem cell research and itspotential application in regenerative medicine. INDICE: Section I: Introduction 1. Current Thoughts on the Therapeutic Potential of Stem Cell Pranela Rameshwar Section II: Stem Cell Study in Lower Organism 2. A Unique FACS Method to Isolate Stem Cells in Planarian Tetsutaro Hayashi and Kiyokazu Agata 3. Identification of Neural Stem Cells in the Drosophila Larval Brain Mo Weng, Hideyuki Komori, and Cheng-Yu Lee 4. Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut Shree Ram Singh, Manoj K. Mishra, Steven X. Hou, and Maduri Kango-Singh Section III: Stem Cell Study in Murine and Human Model 5. Developing a Quantitative In Vivo Tissue Reconstitution Assay to Assess the Relative Potency of Candidate Populations of Mouse Oesophageal Epithelial Cells Daniel Croagh, Rick Redvers, Wayne A. Phillips,and Pritinder Kaur 6. Identification, Isolation, and Culture of Intestinal Epithelial Stem Cells from Murine Intestine Gracz, A.D., Puthoff, B.J., Magness,S.T. 7. Isolation and Characterization of Distal Lung Progenitor Cells Barbara Driscoll, Alex Kikuchi, Allison N. Lau, Jooeun Lee, Raghava Reddy, Edwin Jesudason, Carla F. Kim,?David Warburton 8. Transplantation of Mouse Fetal Liver Cells for Analyzing the Function of Hematopoietic Stem and Progenitor Cells Kristbjorn Orri Gudmundsson, Steven W. Stull, and Jonathan R. Keller 9. Convenient and Efficient Enrichment of the CD133+ Liver Cells From Rat Fetal Liver as a Source of Liver Stem/Progenitor Cells Weihui Liu, Nan You, Kefeng Dou 10. Assessing the Potential Clinical Utility of Transplantations of Neural and Mesenchymal Stem Cells for Treating Neurodegenerative Diseases Laurent Lescaudron, C.Boyer, Virginie Bonnamain, KD. Fink, X. Lévêque, J. Rossignol, V. Nerrière-Daguin, AC Malouet, F. Lelan, N.D Dey, D. Michel-Monigadon, M. Lu, I. Neveu, S. von Hörsten, P. Naveilhan and GL Dunbar 11. Functional Identification of Neural Stem Cell-Derived Oligodendrocytes Sofia Grade, Fabienne Agasse, Liliana Bernardino, João O. Malva 12. Stem/Progenitor Cells in Murine Mammary Gland: Isolation and Functional Characterization Abhik Bandyopadhyay, QiaoxiangDong and Lu-Zhe Sun 13. A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-Cultures Steven J. Greco, Shyam A. Patel,Pranela Rameshwar 14. Isolation, Culture and Osteogenic/chondrogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells Susanne Grässel, SabineStöckl, Zsuzsa Jenei-Lanzl 15. Obtaining Freshly Isolated and Cultured Mesenchymal Stem Cells from Human Adipose Tissue Andrew C. Boquest and Philippe Collas 16. Collection, Processing and Banking of Umbilical Cord Blood Stem Cellsfor Transplantation and Regenerative Medicine Michael S. Badowski and David T. Harris 17. Generation of Functional Islets from Human Umbilical Cord and Placenta Derived Mesenchymal Stem Cells Sachin Kadam, Vijayendran Govindasamy, and Ramesh Bhonde 18. Isolation and Characterization of Human Prostate Stem/Progenitor Cells Changyong Guo, Baohui Zhang, and Isla P. Garraway 19. Isolationand Expansion of Adult Cardiac Stem/Progenitor Cells in the Form of Cardiospheres from Human Cardiac Biopsies and Murine Hearts Isotta Chimenti, Roberto Gaetani, Lucio Barile, Elvira Forte, Vittoria Ionta, Francesco Angelini, Giacomo Frati, Elisa Messina, Alessandro Giacomello. 20. Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells Into Cardiomyocytes Anke M. Smits, Angelique A. van Oorschot and Marie-José Goumans 21. Isolation, Expansion and Characterization of Human Islet-Derived Progenitor Cells Mugdha V. Joglekarand Anandwardhan A. Hardikar 22. Isolation and Characterization of ResidentMesenchymal Stem Cells in Human Glomeruli Stefania Bruno and Giovanni Camussi 23. Endothelial Colony Forming Progenitor Cell Isolation and Expansion Nicole A. Hofmann, Andreas Reinisch and Dirk Strunk 24. Isolation and Characterization of Stem Cell-Enriched Human and Canine Hair Follicle Keratinocytes ManabuOhyama and Tetsuro Kobayashi 25. Human Salivary Gland Stem Cells: Isolation,Propagation and Characterization Silke Schwarz and Nicole Rotter 26. Identification, Isolation, Characterization and Banking of Human Dental Pulp Stem Cells Virginia Tirino, Francesca Paino and Gianpaolo Papaccio 27. Isolation and Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human Skin Hsing-I Huang and Chung-Zu Wu 28. Immortalization of Human Mesenchymal Stromal Cells with Telomerase and Red Fluorescence Protein Expression Chao-Ling Yao and Shiaw-Min Hwang 29. Genetic Modification of Mesenchymal Stem Cells Andréia Escosteguy Vargas, Melissa Medeiros Markoski, Andrés Delgado Cañedo, Flávia Helena da Silva and Nance Beyer Nardi 30. Methodology, Biology and Clinical Applications of Human Mesenchymal Stem Cells Melissa Camassola, Luisa Maria Gomes de Macedo Braga, Pedro Cesar Chagastelles and Nance Beyer Nardi 31. In Vitro Production of Enucleated Red Blood Cells from Hematopoietic Stem and Progenitor CellsKenichi Miharada and Yukio Nakamura 32. Methods for Cancer Stem Cell Detection and Isolation Virginia Tirino, Vincenzo Desiderio, Francesca Paino and Gianpaolo Papaccio 33. Biocompatible Nanoparticle Labelling of Stem Cells and their Distribution in Brain Ashish K. Rehni, Thakur Gurjeet Singh, MansiChitkara, and I. S. Sandhu
- ISBN: 978-1-61779-814-6
- Editorial: Humana
- Encuadernacion: Cartoné
- Fecha Publicación: 31/05/2012
- Nº Volúmenes: 1
- Idioma: Inglés