RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem ofdelivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recentadvances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA's unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular Biology™ series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function,biogenesis, and interference with virus infection. Unparalleled diversity of siRNA delivery and imaging strategies covered in a single volume Details the strategies that predict the design of effective siRNA with minimum side effectsFully explains miRNA biogenesis and how to design microRNA-based vectors and to apply them in vivo Explores the clinical potential of siRNA in human diseases, and describes the first application in patients with cancer INDICE: From the contents Methods for Selecting Effective siRNA Sequences by using Statistical and Clustering Techniques.- Deciphering the Code of Innate Immunity Recognition of siRNAs.- Peptide Targeted Delivery of Antisense Oligonucleotides and siRNAs into Mammalian Cells.- Local and Systemic Delivery of siRNAs for Oligonucleotides Therapy.- Imaging of siRNA Delivery and Silencing.- Recent Advances in Magnetofection and Its Potential to Deliver siRNAs in vitro.- In vitro and in vivo Gene Silencing by Transkingdom RNAi (tkRNAi).- Bacterial Delivery of siRNAs: A New Approach to Solid Tumor Therapy.- The Therapeutic Potential of LNA-modified siRNAs: Reduction of Off-target Effects by Chemical Modification of the siRNA Sequence.- pSM155 and pSM30 Vectors for miRNA andshRNA Expression.- Targeting Oncogenes with siRNAs.- Targeting Stromal-cancerCell Interactions with siRNAs.- Therapeutic Potential of siRNA-mediated Targeting of Urokinase Plasminogen Activator, Its Receptor, and Matrix Metalloproteinases.
- ISBN: 978-1-60327-546-0
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 435
- Fecha Publicación: 01/11/2008
- Nº Volúmenes: 1
- Idioma: Inglés