In Acute Leukemia: Methods and Protocols, expert researchers bring together awide range of state-of-the-art laboratory methods and detailed protocols thatare useful for both clinical and basic research scientists working on the disease. The volume provides techniques for prenatal backtracking of leukemic clone, molecular diagnosis, detection of genome-wide genetic abnormalities and profiling, identification of unknown fusion genes, monitoring of minimal residual diseases, disease modeling using murine and human primary hematopoietic cells, studying of normal and malignant hematopoiesis, identification of interacting partners with leukemia associated oncoproteins, and global characterizationof genome-wide epigenetic changes in leukemic cells. Written in the highly successful Methods in Molecular Biology™ series format, the convenient chapters contain brief introductions, lists of the necessary materials, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding knownpitfalls. Comprehensive global analyses of cancer genome, epigenome and expression profiling of leukemic cells Inclusive guides to disease modeling using normal murine and human primary hemateopoietic cells as well as patient samplesDetailed protocols to characterize critical interacting partners, downstream targets and epigenetic functions of leukemia associated transcription factors INDICE: From the contents An Overview: From Discovery of Candidate Mutations to Disease Modelling and Transformation Mechanisms of Acute Leukemia.- Backtracking of Leukemic Clones to Birth.- Fluorescence in situ Hybridization (FISH) as a Tool for the Detection of Significant Chromosomal Abnormalities in Childhood Leukaemia.- Specialized Fluorescence in situ Hybridization (FISH) Techniques for Leukemia Research.- LDI-PCR: Identification of Known and Unknown Gene Fusions of the Human MLL Gene.- Panhandle PCR Approaches to Cloning MLL Genomic Breakpoint Junctions and Fusion Transcript Sequences.- MRD Detection in Acute Lymphoblastic Leukemia Patients Using Ig/TCR Gene Rearrangements as Targets for Real-Time Quantitative PCR.- Array-Based Comparative Genomic Hybridization as a Tool for Analyzing the Leukemia Genome.- Application of SNP Genotype Arrays to Determine Somatic Changes in Cancer.- Retroviral/Lentiviral Transduction and Transformation Assay.
- ISBN: 978-1-58829-989-5
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 390
- Fecha Publicación: 01/04/2009
- Nº Volúmenes: 1
- Idioma: Inglés