Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leadsto susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials andreagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls. Provides an intuitive reference volume, written by leading international research groups, for key in vitro and in vivo models used in the study of Type II diabetes Presents a modular approach to experimental design, covering model biological systems, detection technologies, and approaches to data analysis INDICE: From the contents Selecting the ‘Right’ Mouse Model for Metabolic Syndrome and Type 2 Diabetes Research.- Nutritional Models of Type 2 Diabetes Mellitus.- The Isolation and Purification of Rodent Pancreatic Islets of Langerhans.- The Measurement of Insulin Secretion from Isolated Rodent Islets of Langerhans.- The Incubation and Monitoring of Cell Viability in Primary Rat Islets of Langerhans and Pancreatic ß-Cell Lines.- In Vitro Culture of Isolated Islets of Langerhans: Analysis of Function.- Single Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine Cells.- Laser Capture Microdissection of Human Pancreatic b –Cells and RNA Preparation for Gene Expression Profiling.- In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like Cells.- The Measurement of GLUT4 Translocation in 3T3-L1 Adipocytes.- Pancreatic Remodeling: Beta Cell Apoptosis, Proliferation and Neogenesis and the Measurement of Beta Cell Mass and of Individual Beta Cell Size.
- ISBN: 978-1-934115-15-2
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 280
- Fecha Publicación: 01/07/2009
- Nº Volúmenes: 1
- Idioma: Inglés