BSL3 and BSL4 agents: proteomics, glycomics, and antigenicity

Edited by leading experts in the EU and the US, this book provides a unique coverage of novel technology approaches for the detection of highly related variants of dangerous agents and novel therapy options. A must-have for all professionals dealing with BSL3 and/or BSL 4 agents INDICE: Preface XIII List of Contributors XV 1 Introduction: Application of Proteomic Technologies for the Analysis of Microbial Infections 1 Jiri Stulik and Patrick Butaye 1.1 Introduction 1 1.2 Search for New Factors of Virulence and Potential Diagnostic Markers 2 1.3 Search for New Vaccine Candidates 3 1.4 Analysis of Post-Translational Modifications of Bacterial Proteins and ProteinProtein Interactions 3 1.5 Conclusions 5 References 5 Part One Basic Proteomic Methods 7 2 Separation of Proteins and Peptides 9 Ludovit Skultety 2.1 Introduction 9 Acknowledgment 24 References 24 3 Basic Mass Spectrometric Approaches 29 Lenka Hernychova and Martin Hubalek 3.1 Introduction 29 3.2 Ionization 30 3.3 Mass Analyzers 32 3.4 Protein Identifi cation 37 3.5 Conclusion 39 Acknowledgments 40 References 40 4 Quantitative Mass Spectrometric Approaches 43 Juraj Lenco and VojteÇch Tambor 4.1 Introduction 43 4.2 iTRAQ Analysis of Bacterial Pathogens 47 References 52 5 BN-PAGE of Microbial Protein Complexes 55 Jiri Dresler and Jana Klimentova 5.1 Introduction 55 5.2 Methods for Studying ProteinProtein Interactions 55 5.3 Blue Native Polyacrylamide Gel Electophoresis56 5.4 Evaluation of BN-PAGE Staining, MS, Western Blotting 60 5.5 BN/SDS-PAGE of ATP Synthase of Francisella tularensis 62 5.6 Conclusion 63 Acknowledgment 63 References 64 6 Analysis of Francisella tularensis Glycoproteins 67 LucieBalonova and Lenka Hernychova 6.1 Introduction to Post-Translational Modifi cations in Prokaryotes 67 6.2 Methodology 68 6.3 Bioinformatics 70 6.4 Application of Glycoproteomic Approach Utilizing ProQ-Emerald and DIG Glycan Kits to Francisella tularensis (F. tularensis) 70 6.5 Results 73 6.6 Conclusion 74 Acknowledgments 76 References 76 Part Two Identification of Proteins and Glycans from Microorganisms as Candidate Molecules for Use in Detection/Diagnosis, Therapy, and Prophylaxis 79 7 Comparative Proteome Analysis of Strains with Differential Virulence 81 Martin Hubalek and Ivona Pávková 7.1 Introduction 81 7.2 Methods 82 7.3 Results 84 7.4 Discussion 86 References 91 8 Analysis of Francisella tularensis Acetonitrile Extracts 95 Lenka Hernychova, Martin Hubalek, andJana Udrzalova 8.1 Introduction 95 8.2 Material and Methods 96 8.3 Results 988.4 Conclusions 102 Acknowledgments 103 References 103 9 Analysis of Culture Filtrate Proteins of Francisella tularensis 107 Klara Konecna, Martin Hubalek,and Lenka Hernychova 9.1 Introduction 107 9.2 Materials and Methods 108 9.3 Results 109 9.4 Discussion 112 Acknowledgments 113 References 113 10 Lipopolysaccharides of Coxiella burnetii: Chemical Composition and Structure, and Their Role in Diagnosis of Q Fever 115 Rudolf Toman and Pavol VadovicÇ 10.1 Introduction 115 10.2 Lipopolysaccharides of C. burnetii 116 10.3 Conclusion 121 Acknowledgments 121 References 121 11 Mimivirus Possesses Anonymous and Unique GeneProducts Endowed for Antigenic Properties 125 Patricia Renesto and Didier Raoult 11.1 Introduction 125 11.2 Material and M

• ISBN: 978-3-527-32780-5
• Editorial: Wiley-VCH
• Encuadernacion: Cartoné
• Páginas: 256
• Fecha Publicación: 21/09/2011
• Nº Volúmenes: 1
• Idioma: Inglés