Plasma Membrane Shaping: Molecular Mechanisms, Theories, and Research Methods summarizes current knowledge on how cells shape their external membrane. Organized in four sections, the book opens with a broad overview of the Plasma Membrane, its composition, usual shapes and substructures, Actin/WASP/arp2/3 structures, BAR domains, and Arikyn repeat domains, Dynamin, and Phospholipid Signaling. Other sections cover the shaping of the Plasma Membrane for transport processes, discussions on Exosomes, macrovesicles, and microsomes, Endosomes, Clathrin-coated pits, caveolae, and other endocytic pits, membrane deformation for cell movement, and the most current dry and wet lab research techniques to investigate cellular membrane shaping. This is an ideal resource for new researchers coming into this area, as well as graduate students. The methods section will be of interest to both microscopists and computer scientists dedicated to the visualization, data collection and analysis of plasma membrane shaping experiments. Covers membrane shaping for both cytosis and cell movement Includes dry and wet lab research methods of plasma membrane shaping Describes the molecular machinery involved with protein and lipid balance in the plasma membrane Presents the coordination of cellular structures involved in cell deformation and motion INDICE: 1. Introduction and overview of the book Part I The Plasma Membrane 2. Membrane lipid composition 3. Membrane shapes and substructures of the plasma membrane 4. Actin/WASP/arp2/3 5. BAR domains 6. Ankyrin repeat domains 7. Dynamin 8. Phospholipid signaling Part II Membrane transport to/from the plasma membrane 9. Exosomes, Microvesicles, and Migrasomes 10. Endosomes 11. Clathrin-coated pits 12. Caveolae, and other endocytic pits 13. Membrane contact sites between plasma membrane and subcellular orgagnelles, and the lipid transport 14. ESCRT Part III The plasma membrane deformation for cell movement 15. Filopodia and Lamellipodia 16. Intracellular organelles during cell migration 17. Cancer metastasis and Podosomes 18. Coupling to Tubulin in cell migration Part IV Microscopic research methods in cellular membrane shaping 19. Electron Microscopy 20. Single molecule imaging 21. New microscopic methods in Cell Biology Part V Reconstitution research methods in cellular membrane shaping 22. Reconstitution of membrane signaling 23. Biomimentic peptide 24. Phase separation of the proteins Part VI Biophysics and cpmputations in cellular membrane shaping 25. Membrane tension, mechanobioogy of cell migration 26. Theroy for membrane remodling 27. 1D migration 28. 2D/3D migration and theory 29. Deep Learning for cell shape analysis 30. Molecular dynamics Part IV: Research methods in Plasma Membrane 13. Deep Learning in Cell Biology: Pang 14. New microscopic methods in Cell Biology : Kiyosue
- ISBN: 978-0-323-89911-6
- Editorial: Academic Press
- Encuadernacion: Rústica
- Páginas: 266
- Fecha Publicación: 01/09/2022
- Nº Volúmenes: 1
- Idioma: Inglés