Basic Life Science Methods: A Laboratory Manual for Students and Researchers

Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource. Provides a practical resource on designing, executing and analyzing experiments and analytical procedures Includes detailed and standardized coverage of basic research methods in the area Presents step-by-step instructions on how to execute a large selection of life sciences experiments INDICE: 1. Calculations of Molarity and Normality 2. Glassware washing and sterilization techniques 3. pH Meter: Its use and calibration 4. Principle and working of Autoclave 5. Principle and working of Centrifuge 6. Principles and working of Laminar 7. Estimation of Total cholesterol (Flegg 1972) 8. Estimation of Creatinine 9. Estimation of blood Urea 10. Estimation of blood glucose By GOD - POD method 11. Determination of Albumin, and Globulin in Plasma 12. Quantitative determination of Homocysteine 13. Estimation of serum bilirubin 14. Estimation of diagnostic enzyme ALT 15. Estimation of diagnostic enzyme AST 16. To Estimate Protein by Lowry's Method 17. Estimation of Reducing sugar by using Dinitro Salicylic Acid (DNS) Method 18. To perform quantitative determination of total protein in Serum/plasma 19. Enzyme Activity Using Starch assay 20. To Estimate Protein by Bradford's Assay 21. To Identify a Protein Sample Using SDS-PAGE 22. Genomic DNA isolation from whole blood 23. PCR for gene amplification 24. Agarose Gel Electrophoresis (AGE) 25. RNA Isolation from Human tissue 26. Real-time polymerase chain reaction (RT-qPCR) 27. Preparation of competent cells by CaCl2 treatment 28. RNA Isolation from Plant tissues 29. DNA isolation from the bacterial culture broth 30. DNA isolation from fungal Mycelium 31. Quantification of the DNA using spectrophotometer 32. Formaldehyde gel electrophoresis of the isolated RNA sample 33. Paper chromatography of different amino acid 34. Separation of plants pigments using Column Chromatography 35. Separation of plant pigment by Thin Layer Chromatography 36. Chromatin Immunoprecipitation (ChIP) Protocol 37. Types of Media 38. Preparation of Nutrient Agar Media 39. Serial Dilution of the Bacterial Cells 40. Spread Plate Method of The Bacterial Cells 41. Streak Plate Method 42. Identified Bacterial Colony in Culture Broth 43. Pour plate method for bacterial colony counting 44. Gram Staining of Bacteria 45. Casein hydrolysis test 46. Antibiotic resistance test by Agar Well Diffusion Method

• ISBN: 978-0-443-19174-9
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 234
• Fecha Publicación: 12/09/2022
• Nº Volúmenes: 1
• Idioma: Inglés