A detailed look at the latest research in non-invasive in vivo cytometry and its applications, with particular emphasis on novel biophotonic methods, disease diagnosis, and monitoring of disease treatment at single cell level in stationary and flow conditions. This book thus covers the spectrum ranging from fundamental interactions between light, cells, vascular tissue, and cell labeling particles, to strategies and opportunities for preclinical and clinical research. General topics include light scattering by cells, fast video microscopy,polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic methods for cellular diagnostics and monitoring of diseasetreatment in living organisms. Also presented are discussions of advanced methods and techniques of classical flow cytometry. INDICE: Perspectives in Cytometry. Novel Concepts and Requirements in Cytometry. Optical Imaging of Cells with Gold Nanoparticle Clusters as Light Scattering. Contrast Agents: A Finite-Difference Time-Domain Approach to the Modeling of Flow Cytometry Configurations. Optics of White Blood Cells: Optical Models, Simulations, Experiments. Optical Properties of Flowing Flood Cells. LaserDiffraction by the Erythrocytes and Deformability Measurements. Characterization of Red Blood Cells Rheological and Physiological State using Optical Flicker Spectroscopy. Digital Holographic Microscopy for Quantitative Live Cell Imaging and Cytometry. Comparison of Immunophenotyping and Rare Cell Detection bySlide-based Imaging Cytometry and by Flow Cytometry. Microfluidic Flow Cytometry: Advancements towards Compact, Integrated Systems. Label-free Cell Classification with Diffraction imaging flow cytometer. An Integrative Approach for Immune Monitoring of Human Health and Disease by Advanced Flow Cytometry Methods. Optical Tweezers and Cytometry. In-vivo image flow cytometry. Instrumentation for In-Vivo Flow Cytometry; A Sickle Cell Anaemia Case Study. Advances in Fluorescence-Based In-vivo Flow Cytometry for Cancer Applications. In-vivo Photothermal and Photoacoustic Flow Cytometry. Optical Instrumentation for the Measurement of Blood Perfusion, Concentration, Oxygenation and Shape In-vivo. Blood Flow Cytometry and Cell Aggregation Study with Laser Speckle. Modificationsof Blood Optical Properties during Photodynamic Reactions in-Vitro and In-vivo.
- ISBN: 978-3-527-40934-1
- Editorial: Wiley-VCH
- Encuadernacion: Cartoné
- Páginas: 734
- Fecha Publicación: 20/04/2011
- Nº Volúmenes: 1
- Idioma: Inglés