Analytical Techniques in Biosciences: From Basics to Applications

Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in research laboratories across the biosciences. The chapters are grouped into two parts. Part I presents chapters that deals with the basic introductory protocols and sample preparatory guidelines for an error free analysis. This section also contains chapters that detail the various analytical techniques such as spectroscopy and mass spectrometry techniques, chromatographic techniques, electrophoretic techniques and molecular weight determination techniques. Part two presents chapters that capture various applied techniques in molecular biology. Techniques for nucleic acid assessments, polymerase chain reaction, DNA sequencing, enzyme application in genetic manipulation, and those used in immunology and Immunochemistry are presented as well. This book draws from experts from key institutions around the globe, who have simplified the chapters in a way that it will be useful to early-stage researchers as well as advanced scientists. The book is also carefully structured and integrated sequentially to aid flow, consistency and continuity. This is a must-have reference for graduate students and researchers in the biosciences. Presents basic analytical protocols, sample preparatory guidelines and conventional techniques in biosciencesDetails the various biochemical and molecular biology techniques including spectroscopic techniques, chromatographical techniques, centrifugation techniques and titrimetric techniquesDiscuses recent advances on novel techniques for screening, identification and characterization of novel moleculesDescribes advanced techniques such as hyphenated techniques, electroanalytical techniques, application of biosensors in biomedical research and automated methods of analysis and robotics INDICE: PART I: BASIC CONCEPTS, PREPARATION AND ANALYTICAL TECHNIQUES 1. Biochemical Analysis, Sampling and Sample Procedures 2. Sample Preparation Techniques 3. Centrifugation Techniques 4. Electrophoretic techniques 5. Chromatographical Techniques 6. Titrimetry 7. Spectroscopy and Mass Spectrometry 8. Hyphenated Techniques 9. Molecular Weight Determination 10. Rheology 11. Electroanalytical Techniques 12. Biosensors 13. Application of Radioisotopes in Bioassays 14. Automated Methods of Analysis and Robotics 15. Enzyme Assays and Protocols 16. Microbial Techniques and Methods: Microscopy PART II: MOLECULAR BIOLOGY TECHNIQUES 17. Nucleic Acid Analytical Techniques 18. Application of Polymerase Chain Reaction in Molecular Biology 19. DNA sequencing 20. Application of Enzymes in Genetic Manipulation 21. Immunology and Immunochemistry 22. Production of Antibodies 23. Preparation of Pure Immunoglobulins 24. Immunochemical Techniques I 25. Immunochemical Techniques II

• ISBN: 978-0-12-822654-4
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 500
• Fecha Publicación: 01/07/2021
• Nº Volúmenes: 1
• Idioma: Inglés