Free radicals and antioxidant protocols

Comprised of twenty-seven chapters contributed by world-renowned experts in the field Fully updated from the first edition to offer cutting-edge methods for the rapid detection of RONs and redox stress Includes in-depth analysis of natural and synthetic antioxidants, and also of DNA oxidation, oxidative lipidomics, and biomarkers INDICE: In Vivo Imaging of Free Radicals and Oxygen.- In Vivo Measurement of Tissue Oxygen using Electron Paramagnetic Resonance Spectroscopy with Oxygen-sensitive Paramagnetic Particle, Lithium Phthalocyanine.- Measurement of Plasma Nitrite by Chemiluminescence.- Determination of Glutathione, MitochondrialTransmembrane Potential, and Cytotoxicity in H9c2 Cardiomyoblasts Exposed to Reactive Oxygen and Nitrogen Species.- Phenolic Acids and Flavonoids: Ocurrence and Analytical Methods.- Design, Synthesis, and Action of Antiatherogenic Antioxidants.- Preparation of Drug-Loaded Polymeric Nanoparticles and Evaluationof the Antioxidant Activity against Lipid Peroxidation.- Nanoparticle and Iron Chelators as a Potential Novel Alzheimer Therapy.- Synthesis and Characterization of Polymer Nanocarriers for the Targeted Delivery of Therapeutic Enzymes.- Assessment of Antioxidant Activity of Eugenol In Vitro and In Vivo.- The Generation of Stable Oxidative Stress-Resistant Phenotypes in Chinese Hamster Fibroblasts Chronically Exposed to Hydrogen Peroxide or Hyperoxia.- A Simple Method for Effective and Safe Removal of Membrane Cholesterol from Lipid Rafts inVascular Endothelial Cells: Implications in Oxidant-Mediated Lipid Signaling.- Superoxide Dismutase - A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile Dysfunction.- Assessing the Reductive Capacity of Cells by Measuring the Recycling of Ascorbic and Lipoic Acids.- Biomarkers of Oxidative Stress: Methods and Measures of Oxidative DNA Damage (COMET Assay) and Telomere Shortening.- Simultaneous Analysis of Mutiple Redox-Sensitive and Apoptotic Gene Expression in Hypothalamic Neurons Exposed to Cholesterol Ozonation Products.- Redox Homeostasis and Cellular Stress Response in Aging and Neurodegeneration.- Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Lung for Pulmonary Hypertension.- A General Method for Quantifying Sequence Effects on Nucleobase Oxidation in DNA.- Analysis of Urinary 8-Oxo-7,8-dihydro-2’-deoxyguanosine by Liquid Chromatography-Tandem Mass Spectrometry.- Oxidative Lipidomics of Apoptosis: Quantitative Assesment of Phospholipid Hydroperoxides in Cells and Tissues.- Simultaneous Analysis of Multiple Lipid Oxidation Products in vivo by Liquid Chromatographic-Mass Spectrometry (LC-MS).- Lipoxygenase-Catalyzed Phospholipid Peroxidation: Preparation, Purification, and Characterization of Phosphatidylinositol Peroxides.- Oxidized Low Density Lipoprotein.- Detection and Localization of Markers of Oxidative Stress by In Situ Methods: Application in the Study of Alzheimer Disease.- Enzyme Immunoassay of Isoprostanes.- Application of Membrane Extraction with Sorbent Interface for Breath Analysis.

• ISBN: 978-1-58829-710-5
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 480
• Fecha Publicación: 01/03/2010
• Nº Volúmenes: 1
• Idioma: Inglés