Since the discovery of DNA structure and throughout the ensuing ‘DNA era’, the field of DNA replication has expanded to cover a vast number of experimentalsystems. In DNA Replication: Methods and Protocols, expert researchers present a collection of techniques and approaches used to investigate DNA replication with an emphasis on the most recent technological developments. Beginning with several informative introductory review chapters, this extensive volume is organized for clarity while fully encouraging innovation by the mixing of methods to create new techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Presents a collection of methods described by the experts in the field of DNA replication Contains overview chapters as an introduction to the field of DNA replication and as a guide in selecting the methods to use Covers techniques for various biological systems, including viral, bacterial, yeast andmammalian DNA replication Provides detailed, step-by-step protocols for setting up a technique INDICE: From the contents Part I: Reviews. Part II: Methods for Analysis of Replication of a Single Locus. Part III: Genome-Wide Analysis Methods. Part IV: Biochemistry and Biophysics Methods. Part V: Cell Biology and Genetic Methods. Part VI: DNA Fiber Analysis Methods.
- ISBN: 978-1-60327-814-0
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 585
- Fecha Publicación: 01/02/2009
- Nº Volúmenes: 1
- Idioma: Inglés