Despite various difficulties, the field of gene therapy, particularly with regard to cancer, has accumulated a tremendous amount of vital pre-clinical and clinical data. Gene Therapy of Cancer: Methods and Protocols, Second Edition fully updates the first edition with expert coverage of established and novel protocols involving both experimental and clinical approaches to cancer gene therapy. This state-of-the-art volume contains overviews of new concepts and strategies with chapters on regulatory and ethical issues, developments, problemsand possible limitations of design and production of gene therapeutics as well as translational issues. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Explores nonviral and viral vectors and relevant gene transfer/delivery systems Includes methods and backgrounds of experimental and clinical gene therapy approaches Covers the important issue of vector design and vector productionfor clinical applications Features the current regulatory and ethical aspectsin translating experimental approaches into clinical application of gene therapy to patients INDICE: From the contents The Development of Gene Therapy: From Monogenic Recessive Disorders to Complex Diseases such as Cancer.- Designing Adenoviral Vectors for Tumor-Specific Targeting.- Analysis of HSV Oncolytic Virotherapy in Organotypic Cultures.- Use of Minicircle Plasmids for Gene Therapy.- Transposable Elements as Plasmid-Based Vectors for Long-Term Gene Transfer into Tumors.- Designing Plasmid Vectors.- Development of Bacterial Vectors for Tumor-Targeted Gene Therapy.- Electroporative Gene Transfer.- Gene Gun Delivery Systemsfor Cancer Vaccine Approaches.- Ultrasound-Mediated Gene Transfection.- Nonviral Jet-Injection Technology for Intratumoral in vivo Gene Transfer of Naked DNA.- Methods for Constructing and Evaluating Anti-Tumor DNA Vaccines.- Immunity of Lentiviral Vector-Modified Dendritic Cells.- Saporin Suicide Gene Therapy.- Utilizing In Vivo Biopanning for the Development of Radiation-Guided Drug Delivery Systems.
- ISBN: 978-1-934115-85-5
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 670
- Fecha Publicación: 01/04/2009
- Nº Volúmenes: 1
- Idioma: Inglés