Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of thefetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as wellas protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, listsof the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Practical and authoritative, Mouse Cell Culture: Methods and Protocols servesas an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders. Features methods involving a wide variety of tissue types in one convenient volume Written by leading experts in an easy-to-use step-by-step format with notes providing practical, straight-from-the-lab knowledge and experiences Coversprotocols with fetal tissues and stem cells as well as cultures of isolated cell types for the study of developmental processes and homogeneous cell populations, respectively INDICE: The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies.- Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures.- Embryonic Skeletal Muscle Micro-Explant Culture and Isolation of Skeletal Muscle Stem Cells.- The Embryonic Kidney: Isolation, Organ Culture, Immunostaining, and RNA Interference.- Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium or Mesenchyme Under Chemically Defined Conditions as a System toEvaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation.- Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine.- Isolation and Culture of Embryonic Pancreas and Liver.-Isolation and Culture of Mouse Satellite Cells.- Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures.- Short and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes.- Isolation, Culture, and Analysis of Mouse Mammary Epithelial Cells.- Isolation and Culture of Mouse Pancreatic Islets for ex vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes.- Isolation and Culture of Adult Mouse Hepatocytes.- Isolation and Culture of Mouse Intestinal Cells.- Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse.- The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry.- Cell Culture of Primary Cerebellar Granule Cells.- Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain.
- ISBN: 978-1-58829-772-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 246
- Fecha Publicación: 01/05/2010
- Nº Volúmenes: 1
- Idioma: Inglés