Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells, 2nd Edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproduciblelaboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. Following the Methods inMolecular Biology™ series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls. Focus on clinical and translational relevance INDICE: From the contents Definitions and Criteria for Stem Cells.- NeuralStem Cells and the Future Treatment of Neurologic Diseases: Raising the Standard.- Neural Differentiation of Human Embryonic Stem Cells.- Generating Neurons from Stem Cells.- Isolation of Neural Stem and Precursor Cells from Rodent Tissue.- Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization.- Adult Neural Stem Cells.- Olfactory Ensheathing Cells: Isolation and Culture from the Olfactory Bulb and Olfactory Epithelium.- Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property.- Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood.- Production of Neurospheres from CNS Tissue.- Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells.- Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures.
- ISBN: 978-1-58829-846-1
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 480
- Fecha Publicación: 01/04/2008
- Nº Volúmenes: 1
- Idioma: Inglés