Divided into three volumes, Micropropagation of Orchids Third Edition retains the exhaustive list of micropropagation protocols for many genera and updates each section to include new and/or revised information about: Culture media and vessels Techniques and procedures for both orchids which were previously cultured and for those which were not Plant hormones and growth regulators Media components Methods for tissue decontamination Historical information Procedures for the cultivation for plantlets which have been removed from flasks Sources of light and illumination methods Written by two globally acknowledged experts in the field, the third edition of this definitive text on the micropropagation of orchids is a detailed and comprehensive collection of procedures and methods for multiplying orchids, including organ, tissue, and cell culture techniques in vitro and is intended for researchers in plant science and propagation, professional and amateur orchid growers, and plant breeding professionals. Much of the general information about techniques and procedures can be applied to plants other than orchids. INDICE: Preface .Preface to the Second Edition .Preface to the First Edition .Chapter 1: History .Terminology .Origins of Orchid Micropropagation .Plant Hormones and Propagation Additives of Plant Origin .Culture of Tissues and Organs .Early Tissue Culture Attempts .Culture of Stem Tips .First Micropropagation of Orchids .The Second Aseptic Culture of an Orchid Explant .Plant Diseases and Meristems .The Third Aseptic Culture of an Orchid Explant .The Fourth Aseptic Culture of an Orchid Explant .Who Pioneered Micropropagation? .Root Cultures .Leaf Cultures .Stems .Flower Buds, Flowers, Floral Segments, and Reproductive Organs .Inflorescences .A Patent .Doubtful Claims .Mutations .Theft in Vitro .Darkening of Culture Media .Anticontaminants .Cell and Protoplast Culture .Flowering in Vitro, .The Future .Chapter 2: General Outline of Techniques and Procedures .Media Components .Macroelements .Microelements .Iron .Auxins and Anti–auxin .Chitosan .Cytokinins .Gibberellins and Antigibberellin .Abscisic Acid .Ethylene and Ethylene Inhibitor .Amino Acids .Polyol .Polyamines .Phloroglucinol .Jasmonates .Vitamins .Nucleotides and Nucleic Acids .Organic Acids .Banana .Coconut Water .Hydrolysates and Autolysates .Sugars .Anticontaminants .Charcoal .Solvents .Solidifiers .pH .Recipes in this Book .Stock Solutions .State of the Medium .Sterilization .Autoclaves .Filtration .Sterilization in Microwave Ovens .Solvents .Open Flame .Liquids .Surface Decontamination .Gas Sterilization .Preparation of the Medium .Culture Vessels .Culture Conditions .Temperature .Agitation .Illumination .Carbon Dioxide .Placing Plant Material in Culture .Seeds .Tissue Explants .Internal Contaminants .Enzymes for Protoplast Isolation .Work Area .Laminar–flow Hoods .Sterile Rooms .Sterile Boxes .Clean Laboratory Bench .Washing Glassware .Chapter 3: Methods for Specific Genera .Acampe .Aeridachnis .Aerides .Aeridovanda .Anacamptis .Angraecum .Anoectochilus .Ansellia .Arachnis .Arachnostylis .Aranda .Aranthera .Arundina .Ascocenda .Ascocentrum .Ascofinetia .Barlia .Bletilla .Brassavola .Brassia .Brassocattleya .Brassolaeliocattleya, 277 .Bulbophyllum .Burkillara .Caladenia .Calanthe .Catasetum .Cattleya .Caularthron .Cleisostoma .Clowesia .Coelogyne .Cymbidium .Cypripedium .Cyrtopodium .Dactylorchis .Dactylorhiza .Darwinara .Dendrobium, 518 .Disa .Diuris .Doriella .Doritaenopsis .Doritis, 693 .Encyclia .Epidendrum .Epiphronitis .Erycina .Eulophia .Gastrochilus .Geodorum .Goodyera .Grammatophyllum .Habenaria .Hadrolaelia .Hetaeria .Holttumara .Ipsea .Kagawara .Laelia .Laeliocattleya .Liparis .Ludisia (Haemaria) .Luisia .Lycaste .Malaxis .Maxillaria .Miltonia .Mokara .Mormodes .Neofinetia .Neostylis .Neottia .Nervilia .Nigritella .Oberonia .Odontioda .Odontoglossum .Odontonia .Oncidium .Ophrys .Orchis .Ottochilus .Pachystoma .Paphiopedilum .Papilionanda .Papilionanthe, 901 .Phaius .Phalaenopsis .Pholidota .Phragmipedium .Pleione .Pogonia .Ponerorchis .Potinara .Pterostylis .Renades .Renanetia .Renantanda .Renanthera .Restrepia .Rhynchostele .Rhynchostylis .Saccolabium .Sarcanthus .Satyrium .Schomburgkia .Serapias .Sophrolaeliocattleya .Spathoglottis .Spiranthes .Tainia .Thelymitra .Thunia .Trudelia .Vanda .Vandofinetia .Vanilla .Vascostylis .Vuylstekeara, 1292 .Zygopetalum, 1292 .Chapter 4: Summary .Culture Conditions .Media Components .Macroelements .Microelements .Substitutions .Carbohydrates .Amino Acids .Vitamins .Polyols .Hormones .Coconut Water .Bleeding Sap of Birch Trees .Other Complex Organic Additives .Solidifying (Gel–forming) Agents and Alternative Supporting Media .Adsorbents .Applications of Orchid Tissue Culture .Commercialization .Conservation .Patents .Plant Improvement and Biotechnology .Cryopreservation .Physiological Studies .Negative Aspects of Micropropagation .References .Appendix 1: General Information on Supplies, Equipment, Terms, and Reagents .Appendix 2: Sources of Supplies and Equipment .Appendix 3: Some Sites of Interest on the World Wide Web .Appendix 4: Light .Appendix 5: Formulary .Appendix 6: Atomic Weights, Concentrations, Exponents, Greek and Roman Letters, Ions, Measurements, Molecular Weights, Prefixes, Valences, Units, and Solutions .Appendix 7: Additional Information .Appendix 8: Plant Preservative Mixture .Appendix 9: Media .Appendix 10: ASCII Codes .Glossary .Index
- ISBN: 978-1-119-18705-9
- Editorial: Wiley–Blackwell
- Encuadernacion: Cartoné
- Páginas: 2368
- Fecha Publicación: 18/08/2017
- Nº Volúmenes: 1
- Idioma: Inglés