Chemical genomics technology has been steadily improving, delivering new biological probes and drugs, and the explicit use of the term ‘chemical proteomics’ has increased with it, as proteins have always been at the heart of this technology. In Chemical Genomics and Proteomics: Reviews and Protocols , experts in the field present updated reviews of the chemistry of small molecules and their interaction with protein targets as well as detailed protocols that coverdifferent types of ligands, carbohydrates, and lipids. For example, the generation of their protein targets and methods for measuring their interactions iscovered. Written in the highly successful Methods in Molecular Biology™ series format, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and up to date, Chemical Genomics and Proteomics: Reviews andProtocols aims to provide inspiration to those who wish to use chemical genomics and proteomics in their work and develop this young field into full maturity through the incorporation of the new biological and chemical technologies beginning to emerge here. Focuses on the foremost, current technologies being used in the fields of chemical genomics and proteomics. Includes clear and concise laboratory protocols, ready for use. Features expert tips and implementation advice to ensure successful results. INDICE: Overview of Chemical Genomics and Proteomics. Exploring Chemical Space: Recent Advances in Chemistry. In Silico Design of Small Molecules. Surface Plasmon Resonance for Proteomics. Biomimetic Affinity Ligands for Immunoglobulins Based on the Multicomponent Ugi Reaction. Preparation of Photo-Cross-Linked Small Molecule Affinity Matrices for Affinity Selection of Protein Targets for Biologically Active Small Molecules. Profiling Cellular Myristoylation and Palmitoylation Using ?-Alkynyl Fatty Acids. Fluorescence Labels in Kinases (FLiK): A High-Throughput Kinase Binding Assay for the Identification of DFG-Out Binding Ligands. Electrochemical Aptamer Sensor for Small Molecule Assays. Miniaturized, Microarray-Based Assays for Chemical Proteomic Studies of Protein Function. Glycan Microarrays. Resolving Bottlenecks for Recombinant Protein Expression in E. coli. Recombinant Protein Expression in the Baculovirus-Infected Insect Cell System. Systems for the Cell-Free Synthesis of Proteins. Protein Identification by MALDI-TOF Mass Spectrometry.
- ISBN: 978-1-61779-348-6
- Editorial: Humana Press
- Encuadernacion: Cartoné
- Páginas: 220
- Fecha Publicación: 28/11/2011
- Nº Volúmenes: 1
- Idioma: Inglés