As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Protocols, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction,and protein function within these living cells. This versatile volume updatesestablished techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Explores a wide variety of model systems, including human, mouse, sea urchin, starfish, zebrafish, and Xenopus oocytes, transformed Caenorhabditis elegans, and tissue culture cells Contains detailed methods easily adaptable to virtually any system Expands the capabilities of cellular and molecular biology laboratories and provides scientists with a valuable mechanistic understanding INDICE: From the contents Expression of Exogenous mRNA in Xenopus laevis Embryos for the Study of Cell Cycle Regulation.- Use of Luciferase Chimaera to Monitor PLC Expression in Mouse Eggs.- Analysis of 14-3-3 Family Member Function in Xenopus Embryos by Microinjection of Antisense Morpholino Oligos.- A Microinjectable Biological System, the Xenopus Oocyte, as an Approach to Understanding Signal Transduction Protein Function.- Combining Microinjection and Immunoblotting to Analyze MAP Kinase Phosphorylation in Single Starfish Oocytes and Eggs.- Analysis of Signaling Pathways in Zebrafish Development by Microinjection.- Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complimentary Approaches to Study Protein Function.- DNA Delivery by Microinjection for the Generation of Recombinant Mammalian Cell Lines.- Bacteriophage fC31 Integrase Mediated Transgenesis in Xenopus laevis forProtein Expression at Endogenous Levels.
- ISBN: 978-1-58829-884-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 225
- Fecha Publicación: 01/02/2009
- Nº Volúmenes: 1
- Idioma: Inglés