Assay development is a practice that involves many disciplines (biology, chemical instrumentation, statistics, analytical chemistry, etc.). There are many scientists practicing assay development in biotechnology and pharmaceutical companies. However, most of the scientists practicing assay development have a biological background and need to integrate several sciences in this interdisciplinary function. This book integrates all of the fields in assay development so that the readers will have a good understanding of both the fundamentals and techniques involved in carrying out an assay project from beginning to end in both a biotechnology/drug discovery setting, where one might be testing a specific new drug candidate, or in a platform setting, where one might be tryingto set up a universal bioassay system that can be used repeatedly for variouscompounds. The book begins with a discussion assay development basics, reviewing measurement, instrumentation, and strategies for high throughput screeningand preclinical drug development. A treatment of assays for purified protein follows, addressing proteases, kinases, and principles that can be applied to the any protein. Cell-based assays are treated next, followed by high-throughput screening. Good Practices are addressed in the penultimate section, and thebook ends with a section on Case Studies which demonstrates how to develop anassay from concept to screen, and an example of developing a novel platform technology to screen kinases.Dr. Ge Wu is Director of Assay Development and Screening at FivePrime Therapeutics. He obtained his Ph.D. in Biochemistry from UCLA in 1992. After postdoctoral work at Harvard Medical School, he joined the faculty at Cornell University Medical College in 1995. In 1997, he joined the then-newly established Automated Biology Department at Merck s off-chip kinase assay product that is now widely used throughout the screening community and derived a theory on substrate conversion with enzymatic reactions that is widely recognized in the globalscreening community as well. Dr. Wu joined FivePrime in 2003 and is responsible for setting up the assay development and screening group to screen therapeutic proteins. He is a leading figure in assay development and screening in biopharmaceutical discovery using primary cell assay and wrote an acclaimed review in this subject.
- ISBN: 978-0-470-19115-6
- Editorial: John Wiley & Sons
- Encuadernacion: Cartoné
- Páginas: 448
- Fecha Publicación: 30/04/2010
- Nº Volúmenes: 1
- Idioma: Inglés